Serving the Society Since 1986
Advanced Search
Volume 21 Issue 5
October 2006
    Citation: YAN Ju-ying*, LU Yi-yu, MAO Hai-yan, FENG Yan, XU Chang-ping, SHI Wen, WENG Jing-qing, LI Min-hong, GONG Li-Ming, GE Qiong, ZHOU Min. Pathogenicity and Molecular Characterization of the H5N1 Influenza Virus Isolated from the First Human Case in Zhejiang Province .VIROLOGICA SINICA, 2006, 21(5) : 430-436.
    shu

    Pathogenicity and Molecular Characterization of the H5N1 Influenza Virus Isolated from the First Human Case in Zhejiang Province

    • 1.

      Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310009,China

    • Available online: 20 September 2006
    • In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) antibody in serum samples was assayed. The results showed that RNA extracted from the tracheal aspirate specimen was positive for H5N1 avian influenza virus and influenza virus type A. The H5N1 avian influenza virus was named A/Zhejiang/16/06 (H5N1). The titers of HI antibody for H5N1 avian influenza virus were 1: 320 and 1: 640, respectively. Both the pathogenic and serologic tests confirmed the human case of H5N1 avian influenza virus infection. The results of HA (hemagglutination) gene sequencing revealed that there were multiple basic amino acids at the cleavage site of HA gene associated with highly pathogenic H5 avian virus. The HA、NA、PB2、NP、M and NS genes were all of avian origin and were very similar to that of H5N1 avian influenza virus isolated from Fujian and Anhui provinces in 2005. However, there were obviously differences compared with the strains isolated from Vietnam、Thailand and Hong Kong in 1997.
    • 加载中
    • 加载中
    PDF

    Article Metrics

    Article views(4385) PDF downloads(814) Cited by()

    Related
    Proportional views

      Pathogenicity and Molecular Characterization of the H5N1 Influenza Virus Isolated from the First Human Case in Zhejiang Province

      • 1. Center for Disease Control and Prevention of Zhejiang Province, Hangzhou 310009,China

      Abstract: In order to confirm the etiology of the first suspected human case of the highly-pathogenic avian influenza virus and to perform the pathogenicity studies, a tracheal aspirate specimen was tested by RT-PCR, Real-time PCR and virus isolation. In addition, the HI (hemagglutination-inhibition) antibody in serum samples was assayed. The results showed that RNA extracted from the tracheal aspirate specimen was positive for H5N1 avian influenza virus and influenza virus type A. The H5N1 avian influenza virus was named A/Zhejiang/16/06 (H5N1). The titers of HI antibody for H5N1 avian influenza virus were 1: 320 and 1: 640, respectively. Both the pathogenic and serologic tests confirmed the human case of H5N1 avian influenza virus infection. The results of HA (hemagglutination) gene sequencing revealed that there were multiple basic amino acids at the cleavage site of HA gene associated with highly pathogenic H5 avian virus. The HA、NA、PB2、NP、M and NS genes were all of avian origin and were very similar to that of H5N1 avian influenza virus isolated from Fujian and Anhui provinces in 2005. However, there were obviously differences compared with the strains isolated from Vietnam、Thailand and Hong Kong in 1997.

        HTML

      Relative (20)

      目录

      This journal is a member of, and subscribes to the principles of, the Committee on Publication Ethics (COPE)

      鄂ICP备05001977号

         

      鄂公网安备 42010602003674号

      Copyright © 2019 Virologica Sinica. All rights reserved

      /

      DownLoad:  Full-Size Img  PowerPoint
      Return
      Return