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Volume 21 Issue 5
October 2006
    Citation: SHI Li-li, GU Chao-jiang, ZHANG Qian, ZHANG Wei-Ying, LI Yong, LU Bin, HE cheng, QU San-fu, ZHENG Cong-yi*. Application of an Improved Real-time TaqMan RT-PCR in FMDV Detection .VIROLOGICA SINICA, 2006, 21(5) : 449-453.
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    Application of an Improved Real-time TaqMan RT-PCR in FMDV Detection

    • 1.

      State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

    • Available online: 20 September 2006
    • In this study, an improved real-time RT-PCR was applied to quantification of Foot-and-mouth disease virus in vivo and in vitro. The results suggested that this method had a high sensitivity with up to less than 10 copies / reaction. Furthermore, this novel method was successfully used to quantify the transcriptional level of FMDV-3D gene in transfected BHK-21 cells and the resultant concentration of RNA level was up to 6.9×10 4 copies/μL. Compared with SYBR GreenⅠRT-PCR, six of eight samples testing the FMDV RNA copy number was up to 6.7-fold higher when determined by TaqMan RT-PCR, indicating that the improved method shows a high sensitivity in virus detection.
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      Application of an Improved Real-time TaqMan RT-PCR in FMDV Detection

      • 1. State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China

      Abstract: In this study, an improved real-time RT-PCR was applied to quantification of Foot-and-mouth disease virus in vivo and in vitro. The results suggested that this method had a high sensitivity with up to less than 10 copies / reaction. Furthermore, this novel method was successfully used to quantify the transcriptional level of FMDV-3D gene in transfected BHK-21 cells and the resultant concentration of RNA level was up to 6.9×10 4 copies/μL. Compared with SYBR GreenⅠRT-PCR, six of eight samples testing the FMDV RNA copy number was up to 6.7-fold higher when determined by TaqMan RT-PCR, indicating that the improved method shows a high sensitivity in virus detection.

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