Citation: MENG Fan-lei, DIAO You-xiang*, WANG Hui, LIU Fang-na, WANG Ni. Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus .VIROLOGICA SINICA, 2006, 21(5) : 468-471.

Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

  • Available online: 20 September 2006
  • A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9%~91.4%, and the identity of the deduced amino acids was 75.8%~77.5%. The results showed that there were major variatons in the N gene of 793/B.

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    Cloning and Sequence Analysis of the Nucleocapsid Gene of a Variant Strain (793/B) of Avian Infectious Bronchitis Virus

    • 1. College of Animal Science and Technology, Shandong Agricultural University, Tai,an 271018 China

    Abstract: A pair of primers were designed and synthesized based onthe genomic sequences of the Nucleocapsid gene of IBV in Genbank. The gene encoding the nucleocapsid protein (N) was amplified by RT-PCR. Sequencing data showed that the complete gene encoding the nucleocapsid of IBV isolate (793/B) consisted of 1229 nucleotides. Sequence analysis showed that 88 point mutations were found in the N gene, and one nucleotide was deleted inform position 991. By comparison with 11 strains in GenBank, the sequence identity was 86.9%~91.4%, and the identity of the deduced amino acids was 75.8%~77.5%. The results showed that there were major variatons in the N gene of 793/B.

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