Complete Sequence Analysis of Genomic S RNA of Capsicum chlorosis Virus Infecting Peanuts in China
Abstract: A new tospovirus was discovered in infected peanut plants based on complete sequencing and analysis of its genomic S RNA. It was characterized as a new strain of Capsicum chlorosis virus (CaCV) and was named China peanut (CP) strain. The total sequence of S RNA of CaCV-CP was 3399 nucleotide (nt) and contained two open reading frames (ORFs) with an ambisense arrangement. ORF1 was 1320nt encoding a 49.9 kDa nonstructural (NSs) protein. ORF2 is 828nt encoding a 30.7 kDa nucleocapsid (N) protein. The N gene of CaCV-CP shared 84.7%~86.4% and 92.4%~93.1% identity with that of CaCV strains from Thailand and Australia at the nucleotide and amino acid levels, respectively. This is the first report of complete sequence of CaCV S RNA and occurrence of CaCV in China.