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Volume 21 Issue 6
December 2006
    Citation: LI Jian-qiang, LIU Ji-xing, CHENG Jie, LAN Xi, HU Yong-hao, WU Run. Cloning and Characterization of the Polymerase Gene of Porcine Coronavirus .VIROLOGICA SINICA, 2006, 21(6) : 551-555.
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    Cloning and Characterization of the Polymerase Gene of Porcine Coronavirus

    • 1.

      1. Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

    • 2.

      College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

    • Corresponding author: LIU Ji-xing, 
    • Available online: 20 November 2006
    • The polymerase gene, a non-structural gene from strain TS of transmissible gastroenteritis virus (TGEV), was amplified by RT-PCR primers designed based on the Purdue nucleotide sequence in GenBank. The expected 20054 bp product was obtained. The nucleotide sequence of ORF1 of TS shared nucleotide and amino acid identities of 98.8% and 99.0%, respectively with that of strain Pur46-MAD. The identity at the amino acid level for the ORF1 between TS and FIPV, PEDV, HCV299E,SARS was 87%、57%、57%、45%, respectively. RdRp was regarded to have an important role in replication and the results indicated that it was a conserved protein. The data also showed that there was a ribosomal slippage site UUUAAAC and three stem-loop structures in the ORF1a and ORF1b overlapping regions.
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      Cloning and Characterization of the Polymerase Gene of Porcine Coronavirus

        Corresponding author: LIU Ji-xing,
      • 1. 1. Key laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
      • 2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China

      Abstract: The polymerase gene, a non-structural gene from strain TS of transmissible gastroenteritis virus (TGEV), was amplified by RT-PCR primers designed based on the Purdue nucleotide sequence in GenBank. The expected 20054 bp product was obtained. The nucleotide sequence of ORF1 of TS shared nucleotide and amino acid identities of 98.8% and 99.0%, respectively with that of strain Pur46-MAD. The identity at the amino acid level for the ORF1 between TS and FIPV, PEDV, HCV299E,SARS was 87%、57%、57%、45%, respectively. RdRp was regarded to have an important role in replication and the results indicated that it was a conserved protein. The data also showed that there was a ribosomal slippage site UUUAAAC and three stem-loop structures in the ORF1a and ORF1b overlapping regions.

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