Citation: WANG Yan, LEI Sen-Lin, GUO Shu-Fang, GAO Qian, DAI Ying, DONG Chang-Yuan. Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain .VIROLOGICA SINICA, 2006, 21(6) : 556-559.

Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain

  • Corresponding author: DONG Chang-Yuan, 
  • Available online: 20 November 2006
  • When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that BTV-HbC3 could inhibit the Hep-3B cells proliferation moderately, which depended on the concentration of BTV-HbC3 and time.The human Hepatic carcinoma 3B cells infected by BTV-HbC3 had all the characteristic of apoptosis. Our finding indicated that oncolytic way of BTV-HbC3 was selectively replicated in tumor cells and induced apoptosis. It was further shown in our laboratory that normal human cells couldn’t be infected by this kind of virus suggesting that this virus possessed the potential function of killing the human hepatic carcinoma.

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    Apoptosis of the Human Hepatic Carcinoma 3B Cells Induced by Bluetongue Virus HbC3 Strain

      Corresponding author: DONG Chang-Yuan,
    • 1. 1. State Key Laboratory of Virology, Wuhan University School of Medicine, Wuhan 430071, China
    • 2. Research Center for Medicine and Structural Biology, Wuhan University School of Medicine, Wuhan 430071, China

    Abstract: When investigating the effect of BTV-HbC3 on the hepatic carcinoma 3B (Hep-3B) cells , we detected a cytopathic effect (CPE) and inhibition activity of Hep-3B cells infected with BTV-HbC3 by using MTT, the apoptosis indicator using flow cytometry (FCM) and microstructures. The results showed that BTV-HbC3 could inhibit the Hep-3B cells proliferation moderately, which depended on the concentration of BTV-HbC3 and time.The human Hepatic carcinoma 3B cells infected by BTV-HbC3 had all the characteristic of apoptosis. Our finding indicated that oncolytic way of BTV-HbC3 was selectively replicated in tumor cells and induced apoptosis. It was further shown in our laboratory that normal human cells couldn’t be infected by this kind of virus suggesting that this virus possessed the potential function of killing the human hepatic carcinoma.

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