Expression of Envelope Protein VP19 of Penaeus monodon WSSV in E.coli and the Effect against WSSV
Abstract: The vp19 gene of White spot syndrome virus (WSSV) was amplified by PCR with specific primers and subcloned into the prokaryotic expression vector pET32b to get the recombinant plasmid pET32b-vp19. A positive plasmid was transformed into the host cell Origami（DE3）pLysS and the target gene was successfully expressed as a fusion protoen when induced with IPTG. The molecular weight of the engineered protein was about 37kDa as demonstrated by SDS-PAGE and Western-blot. The engineered protein Trx-VP19 purified by Ni2+-column chromatography was injected into crayfish or used as a supplement to feed crayfish. Crayfish vaccinated by intramuscular injection with Trx-VP19 showed lower cumulative mortality due to WSSV compared to vaccination with bacteria expressing the empty vectors.