Citation: JIA Qi-jun, MENG Xiao-lin*, XU Jin-ping, WANG Jian, LU Wei. Expression of Envelope Protein VP19 of Penaeus monodon WSSV in E.coli and the Effect against WSSV .VIROLOGICA SINICA, 2006, 21(6) : 585-588.

Expression of Envelope Protein VP19 of Penaeus monodon WSSV in E.coli and the Effect against WSSV

  • Available online: 20 November 2006
  • The vp19 gene of White spot syndrome virus (WSSV) was amplified by PCR with specific primers and subcloned into the prokaryotic expression vector pET32b to get the recombinant plasmid pET32b-vp19. A positive plasmid was transformed into the host cell Origami(DE3)pLysS and the target gene was successfully expressed as a fusion protoen when induced with IPTG. The molecular weight of the engineered protein was about 37kDa as demonstrated by SDS-PAGE and Western-blot. The engineered protein Trx-VP19 purified by Ni2+-column chromatography was injected into crayfish or used as a supplement to feed crayfish. Crayfish vaccinated by intramuscular injection with Trx-VP19 showed lower cumulative mortality due to WSSV compared to vaccination with bacteria expressing the empty vectors.

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    Expression of Envelope Protein VP19 of Penaeus monodon WSSV in E.coli and the Effect against WSSV

    • 1. State Key Laboratory of Virology, Wuhan University, Wuhan 430072, China

    Abstract: The vp19 gene of White spot syndrome virus (WSSV) was amplified by PCR with specific primers and subcloned into the prokaryotic expression vector pET32b to get the recombinant plasmid pET32b-vp19. A positive plasmid was transformed into the host cell Origami(DE3)pLysS and the target gene was successfully expressed as a fusion protoen when induced with IPTG. The molecular weight of the engineered protein was about 37kDa as demonstrated by SDS-PAGE and Western-blot. The engineered protein Trx-VP19 purified by Ni2+-column chromatography was injected into crayfish or used as a supplement to feed crayfish. Crayfish vaccinated by intramuscular injection with Trx-VP19 showed lower cumulative mortality due to WSSV compared to vaccination with bacteria expressing the empty vectors.

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