Shi-Zhe Xie, Mei-Qin Liu, Ren-Di Jiang, Hao-Feng Lin, Wei Zhang, Bei Li, Jia Su, Fei Ke, Qi-Ya Zhang, Zheng-Li Shi and Xing-Lou Yang. Fish ACE2 is not susceptible to SARS-CoV-2[J]. Virologica Sinica, 2022, 37(1): 142-144. doi: 10.1016/j.virs.2022.01.020
Citation: Shi-Zhe Xie, Mei-Qin Liu, Ren-Di Jiang, Hao-Feng Lin, Wei Zhang, Bei Li, Jia Su, Fei Ke, Qi-Ya Zhang, Zheng-Li Shi, Xing-Lou Yang. Fish ACE2 is not susceptible to SARS-CoV-2 .VIROLOGICA SINICA, 2022, 37(1) : 142-144.  http://dx.doi.org/10.1016/j.virs.2022.01.020

新冠病毒不能利用鱼ACE2入侵细胞

  • 通讯作者: 杨兴娄, yangxl@wh.iov.cn
  • 收稿日期: 2021-08-26
    录用日期: 2022-01-12
  • 最近在冷冻鱼中检测到新冠病毒的报道引起了人们对鱼类在新冠病毒传播中的潜在作用的担忧。 本研究调查了鱼类 ACE2 对新冠病毒的易感性。为了确定这一点,我们用新冠病毒感染了三种鱼细胞系(鲤鱼上皮细胞、草鱼肾脏细胞和蓝鳃太阳鱼细胞)。 此外,我们在 Hela 细胞中表达了五种鱼 ACE2(斑马鱼、罗非鱼、鲶鱼、鲑鱼和虹鳟鱼)并感染了新冠病毒。 通过免疫荧光测定 (IFA) 和定量逆转录聚合酶链反应 (qRT-PCR),在鱼细胞系或 Hela 细胞表达的鱼 ACE2 中未检测到病毒抗原。综上所述,IFA 和 qRT-PCR 结果证实新冠病毒不能与鱼类 ACE2 结合以侵入宿主细胞。

Fish ACE2 is not susceptible to SARS-CoV-2

  • Corresponding author: Xing-Lou Yang, yangxl@wh.iov.cn
  • Received Date: 26 August 2021
    Accepted Date: 12 January 2022
  • The ongoing pandemic of coronavirus disease 2019 (COVID-19) has reshaped our daily life and caused > 4 million deaths worldwide (https://covid19.who.int/). Although lockdown and vaccination have improved the situation in many countries, imported cases and sporadic outbreaks pose a constant stress to the prevention and control of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent responsible for COVID-19, has a positive-sense single-stranded RNA genome of 30 kb (Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, 2020). We and other groups have demonstrated that the SARS-CoV-2 could use the angiotensin-converting enzyme 2 (ACE2) as cell receptor, including orthologs of a broad range of animal species such as human, bats, ferrets, pigs, cats, and dogs (Hoffmann et al., 2020; Zhou et al., 2020; Liu et al., 2021). Although the evolutionary origin of SARS-CoV-2 can be linked to the discoveries of diverse coronaviruses related to SARS-CoV-2 in wild animals such as bats (Zhou et al., 2020; Wacharapluesadee et al., 2021) and pangolins (Liu et al., 2019; Lam et al., 2020), the direct origin of SARS-CoV-2 in humans remains unknown. In China, several sporadic outbreaks of COVID-19 in 2020 were linked to food in cold chain sold at trade markets, including salmon meat (http://www.nhc.gov.cn/xcs/yqtb/list_gzbd.shtml) (Yang et al., 2020). The detection of SARS-CoV-2 RNA on the surface of frozen meat for as long as 20 days has also been reported (Feng et al., 2021). A concern regarding the potential role of fish in SARS-CoV-2 transmission has also been raised. Therefore, we investigated the susceptibility of fish ACE2 to SARS-CoV-2.

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    Fish ACE2 is not susceptible to SARS-CoV-2

      Corresponding author: Xing-Lou Yang, yangxl@wh.iov.cn
    • a CAS Key Laboratory of Special Pathogens, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China
    • b University of Chinese Academy of Sciences, Beijing, 100049, China

    Abstract: The ongoing pandemic of coronavirus disease 2019 (COVID-19) has reshaped our daily life and caused > 4 million deaths worldwide (https://covid19.who.int/). Although lockdown and vaccination have improved the situation in many countries, imported cases and sporadic outbreaks pose a constant stress to the prevention and control of COVID-19. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiologic agent responsible for COVID-19, has a positive-sense single-stranded RNA genome of 30 kb (Coronaviridae Study Group of the International Committee on Taxonomy of Viruses, 2020). We and other groups have demonstrated that the SARS-CoV-2 could use the angiotensin-converting enzyme 2 (ACE2) as cell receptor, including orthologs of a broad range of animal species such as human, bats, ferrets, pigs, cats, and dogs (Hoffmann et al., 2020; Zhou et al., 2020; Liu et al., 2021). Although the evolutionary origin of SARS-CoV-2 can be linked to the discoveries of diverse coronaviruses related to SARS-CoV-2 in wild animals such as bats (Zhou et al., 2020; Wacharapluesadee et al., 2021) and pangolins (Liu et al., 2019; Lam et al., 2020), the direct origin of SARS-CoV-2 in humans remains unknown. In China, several sporadic outbreaks of COVID-19 in 2020 were linked to food in cold chain sold at trade markets, including salmon meat (http://www.nhc.gov.cn/xcs/yqtb/list_gzbd.shtml) (Yang et al., 2020). The detection of SARS-CoV-2 RNA on the surface of frozen meat for as long as 20 days has also been reported (Feng et al., 2021). A concern regarding the potential role of fish in SARS-CoV-2 transmission has also been raised. Therefore, we investigated the susceptibility of fish ACE2 to SARS-CoV-2.

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