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Volume 37 Issue 2
April 2022

    Yali Hao, Siyuan Wang, Mujie Zhang, Qingxue Tang, Canxing Meng, Liping Wang, Qilian Fan, Yaxian Yan, Xiang Xiao and Huahua Jian. Isolation and characterization of a novel linear-plasmid phage from the sediment of the Mariana Trench[J]. Virologica Sinica, 2022, 37(2): 311-313. doi: 10.1016/j.virs.2022.01.022
    Citation: Yali Hao, Siyuan Wang, Mujie Zhang, Qingxue Tang, Canxing Meng, Liping Wang, Qilian Fan, Yaxian Yan, Xiang Xiao, Huahua Jian. Isolation and characterization of a novel linear-plasmid phage from the sediment of the Mariana Trench .VIROLOGICA SINICA, 2022, 37(2) : 311-313.  http://dx.doi.org/10.1016/j.virs.2022.01.022
    shu

    马里亚纳海沟沉积物中一株新型线状质粒噬菌体的分离与鉴定

    • a 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 教育部代谢与发育科学国际合作联合实验室, 上海 200240

    • b 自然资源部第三海洋研究所海洋生物遗传资源重点实验室, 厦门 361005

    • c 上海交通大学农业与生物学院, 上海市兽医生物技术重点实验室, 上海 200240

    • d 南方海洋科学与工程广东省实验室(珠海), 广东 519082

    • 通讯作者： 蹇华哗, jiandy@sjtu.edu.cn
    • 收稿日期： 2021-06-23
      录用日期： 2021-10-20
    • 病毒是自然环境中丰度及多样性最高的生物类群，在深海生物圈的物质能量循环和种群平衡调节方面发挥着重要作用。深渊作为深海生态系统的重要组成部分，但目前从中分离的可培养噬菌体却极少。本研究从马里亚纳海沟8636米水深的沉积物中分离到一株Halomonas属的细菌MT08-1。通过丝裂霉素诱导，分离纯化得到一株线状质粒噬菌体HMP1。电镜形态观察显示，HMP1有一个直径约56 nm的多边形头部及长约181 nm的鞘状尾部，属于肌尾噬菌体科（Myoviridae）。限制性酶切片段多态性分析表明该噬菌体的遗传物质类型为双链DNA。测序结果显示，其基因组大小为38130 bp，G+C含量为59.7%。系统进化树构建表明HMP1与来自海洋表层的ΦHAP-1亲缘关系较近，并与3株海洋来源的线状质粒噬菌体位于同一进化分支。本研究结果填补了目前深渊沉积物来源的可培养噬菌体的空白，为今后极端深渊环境中噬菌体及其宿主的研究提供了材料。

    Isolation and characterization of a novel linear-plasmid phage from the sediment of the Mariana Trench

    • a State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    • b Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China

    • c Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China

    • d Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519082, China

    • Corresponding author: Huahua Jian, jiandy@sjtu.edu.cn
    • Received Date: 23 June 2021
      Accepted Date: 20 October 2021
    • Highlights
      1. HMP1 is the first bacteriophage that was isolated from hadal sediment, the water depth at which HMP1 was isolated is the highest on record up to now.
      2. The isolation of HMP1 extends the habitat of linear plasmid phages from the surface to the deep ocean.
      3. The genomic and morphological features of HMP1 provide hints with regard to the vertical exchange of viral communities in the ocean.
      4. HMP1 and Halomonas sp. MT08-1 contribute a useful phage-host system for in-depth analysis of the life strategy of viruses and their interactions with bacterial hosts in extreme hadal environments.
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      1. Adriaenssens, E.M., Brister, J.R., 2017. How to name and classify your phage: an informal guide. Viruses 9, 70.

      2. Howard-Varona, C., Hargreaves, K.R., Abedon, S.T., Sullivan, M.B., 2017. Lysogeny in nature: mechanisms, impact and ecology of temperate phages. ISME J. 11, 1511–1520.

      3. Hurwitz, B.L., Sullivan, M.B., 2013. The Pacific Ocean virome (POV): a marine viral metagenomic dataset and associated protein clusters for quantitative viral ecology. PLoS One 8, e57355.

      4. Jamieson, A.J., Fujii, T., Mayor, D.J., Solan, M., Priede, I.G., 2010. Hadal trenches: the ecology of the deepest places on Earth. Trends Ecol. Evol. 25, 190–197.

      5. Jian, H., Yi, Y., Wang, J., Hao, Y., Zhang, M., Wang, S., Meng, C., Zhang, Y., Jing, H., Wang, Y., Xiao, X., 2021. Diversity and distribution of viruses inhabiting the deepest ocean on Earth. ISME J. 15, 3094–3110.

      6. Mobberley, J.M., Authement, R.N., Segall, A.M., Paul, J.H., 2008. The temperate marine phage fhap-1 of Halomonas aquamarina possesses a linear plasmid-like prophage genome. J. Virol. 82, 6618–6630.

      7. Ravin, N.V., 2003. Mechanisms of replication and telomere resolution of the linear plasmid prophage N15. FEMS Microbiol. Lett. 221, 1–6.

      8. Ravin, N.V., 2011. N15: the linear phage-plasmid. Plasmid 65, 102–109.

      9. Ravin, N.V., 2015. Replication and maintenance of linear phage-plasmid N15. Microbiol. Spectr. 3. PLAS-0032-2014.

      10. Ravin, V., Ravin, N., Casjens, S., Ford, M.E., Hatfull, G.F., Hendrix, R.W., 2000. Genomic sequence and analysis of the atypical temperate bacteriophage N15. J. Mol. Biol. 299, 53–73.

      11. Shen, M., Yang, Y., Shen, W., Cen, L., McLean, J.S., Shi, W., Le, S., He, X., 2018. A linear plasmid-like prophage of Actinomyces odontolyticus promotes biofilm assembly. Appl. Environ. Microbiol. 84 e01263-18.

      12. Yoshida, M., Yoshida-Takashima, Y., Nunoura, T., Takai, K., 2015. Identification and genomic analysis of temperate Pseudomonas bacteriophage PstS-1 from the Japan trench at a depth of 7000 m. Res. Microbiol. 166, 668–676.

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      10.1016j.virs.2022.01.022-ESM.docx
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      Isolation and characterization of a novel linear-plasmid phage from the sediment of the Mariana Trench

        Corresponding author: Huahua Jian, jiandy@sjtu.edu.cn
      • a State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China
      • b Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, China
      • c Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China
      • d Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai, 519082, China
      • Received Date: 23 June 2021
      • Accepted Date: 20 October 2021

      Abstract: Highlights
      1. HMP1 is the first bacteriophage that was isolated from hadal sediment, the water depth at which HMP1 was isolated is the highest on record up to now.
      2. The isolation of HMP1 extends the habitat of linear plasmid phages from the surface to the deep ocean.
      3. The genomic and morphological features of HMP1 provide hints with regard to the vertical exchange of viral communities in the ocean.
      4. HMP1 and Halomonas sp. MT08-1 contribute a useful phage-host system for in-depth analysis of the life strategy of viruses and their interactions with bacterial hosts in extreme hadal environments.

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