Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus

Liqin Liao; Weiguo Chen; Xiangyu Zhang; Huanmin Zhang; Aijun Li; Yiming Yan; Zi Xie; Hongxing Li; Wencheng Lin; Jingyun Ma; Xinheng Zhang; Qingmei Xie

doi:10.1016/j.virs.2022.01.026

April 2022

Liqin Liao, Weiguo Chen, Xiangyu Zhang, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxing Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang and Qingmei Xie. Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus[J]. Virologica Sinica, 2022, 37(2): 284-294. doi: 10.1016/j.virs.2022.01.026

Citation: Liqin Liao, Weiguo Chen, Xiangyu Zhang, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxing Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang, Qingmei Xie. Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus .VIROLOGICA SINICA, 2022, 37(2) : 284-294. http://dx.doi.org/10.1016/j.virs.2022.01.026

精液细胞外囊泡介导J亚型禽白血病病毒垂直传播

廖立钦 ^a,b,e ,
陈伟国 ^a,b,e,f ,
张翔宇 ^a,e ,
张焕民 ^c ,
李爱军 ^d ,
严一铭 ^a,b,e ,
谢姿 ^a,b,e ,
李鸿鑫 ^a,b,e ,
蔺文成 ^a,b,e,f ,
马静云 ^a,b,e,f ,
张新珩 ^a,b,e,, ,
谢青梅 ^a,b,e,f,,

华南农业大学动物科学学院畜禽健康养殖与疾病防控实验室

通讯作者： 张新珩, xhzhang@scau.edu.cn; 谢青梅, qmx@scau.edu.cn
收稿日期： 2021-06-16
录用日期： 2021-12-27

摘要

J亚型禽白血病病毒（ALV-J）属于致瘤性的逆转录病毒，对全世界的养禽业造成严重的影响。垂直传播是J亚型禽白血病病毒的主要传染途径。大量研究表明，来源于病毒感染的细胞或者体液的细胞外囊泡可作为病毒传播的载体。然而，关于细胞外囊泡在J亚型禽白血病病毒感染中的作用尚未阐明。在本研究中，从ALV-J感染的鸡精液中分离纯化了细胞外囊泡（SE-ALV-J），通过转录组测序、反转录定量PCR和蛋白免疫印迹证明其包含了ALV-J病毒的基因组RNA和病毒蛋白；并证明SE-ALV-J能够感染宿主细胞。更重要的是，通过人工授精的方法，展示了SE-ALV-J可以感染成年母鸡进而介导ALV-J感染后代的垂直传播。总的来说，我们的结果揭示了J亚型禽白血病病毒可利用宿主精液的细胞外囊泡作为垂直传播的新的传播途径，加深了对J亚型禽白血病病毒传播机制的理解。
- 精液细胞外囊泡
- , J亚型禽白血病病毒
- , 感染
- , 垂直传播

Semen extracellular vesicles mediate vertical transmission of subgroup J avian leukosis virus

Liqin Liao ^a,b,e ,
Weiguo Chen ^a,b,e,f ,
Xiangyu Zhang ^a,e ,
Huanmin Zhang ^c ,
Aijun Li ^d ,
Yiming Yan ^a,b,e ,
Zi Xie ^a,b,e ,
Hongxing Li ^a,b,e ,
Wencheng Lin ^a,b,e,f ,
Jingyun Ma ^a,b,e,f ,
Xinheng Zhang ^a,b,e,, ,
Qingmei Xie ^a,b,e,f,,

a Heyuan Branch, Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, College of Animal Science, South China Agricultural University, Guangzhou, 510642, China

Corresponding author: Xinheng Zhang, xhzhang@scau.edu.cn Qingmei Xie, qmx@scau.edu.cn
Received Date: 16 June 2021
Accepted Date: 27 December 2021

Abstract

Subgroup J avian leukosis virus (ALV-J) is a highly oncogenic retrovirus that has been devastating the global poultry industry since the late 1990s. The major infection model of ALV-J is vertical transmission, which is responsible for the congenital infection of progeny from generation to generation. Increasing evidence has suggested that extracellular vesicles (EVs) derived from virus-infected cells or biological fluids have been thought to be vehicles of transmission for viruses. However, the role of EVs in infection and transmission of ALV-J remains obscure. In the present study, semen extracellular vesicles (SE) were isolated and purified from ALV-J-infected rooster seminal plasma (SE-ALV-J), which was shown to contain ALV-J genomic RNA and partial viral proteins, as determined by RNA sequencing, reverse transcription-quantitative PCR and Western blotting. Furthermore, SE-ALV-J was proved to be able to transmit ALV-J infection to host cells and establish productive infection. More importantly, artificial insemination experiments showed that SE-ALV-J transmitted ALV-J infection to SPF hens, and subsequently mediated vertical transmission of ALV-J from the SPF hens to the progeny chicks. Taken together, the results of the present study suggested that ALV-J utilized host semen extracellular vesicles as a novel means for vertical transmission, enhancing our understanding on mechanisms underlying ALV-J transmission.
- Semen extracellular vesicles (SE)
- , Subgroup J avian leukosis virus (ALV-J)
- , Infection
- , Vertical transmission

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10.1016j.virs.2022.01.026-ESM.docx