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. doi: 10.1016/j.virs.2022.02.008
Citation: Minglong Liang, Zongmei Wang, Chuanjian Wu, Sidong Xiong, Ling Zhao, Chunsheng Dong. A single dose of recombinant VSV-RABVG vaccine provides full protection against RABV challenge [J].VIROLOGICA SINICA, 2022, 37(3) : 455-458.  http://dx.doi.org/10.1016/j.virs.2022.02.008

单剂重组水泡性口炎病毒VSV-RABVG疫苗免疫对狂犬病毒RABV感染提供充分保护

  • 由狂犬病病毒(RABV)感染引起的狂犬病仍然是一个严重的全球公共卫生问题,每年造成6万多人死亡。市面上用于人的狂犬病疫苗是通过组织或者鸡鸭胚培养而来,灭活纯化的RABV颗粒。目前,无论是暴露前预防还是暴露后预防都需要多次接种灭活的RABV疫苗。重组水泡性口炎病毒(rVSV)被广泛用作疫苗平台。在本研究中,我们构建了RABV-G囊膜蛋白替换的rVSV并评估了免疫小鼠的抗原特异性体液免疫应答。我们证明单剂量VSV-RABVG鼻内免疫可诱导有效的抗原特异性体液免疫应答,尤其是免疫后4周诱导的病毒中和抗体。更为重要的是,免疫小鼠在受到致病性RABV株DRV-Mexico感染时,与RABV减毒株免疫小鼠(60%)相比,用VSV-RABVG免疫的小鼠100%得到充分保护。总之,我们的研究提供了一种有效且方便的基于VSV的疫苗,在防控狂犬病方面具有潜在的应用价值。

A single dose of recombinant VSV-RABVG vaccine provides full protection against RABV challenge

  • Highlights:
    1. A replication-competent recombinant VSV with RABV-G protein replacement was generated.
    2. Single dose of VSV-RABVG immunization induce potent antigen-specific humoral immune response, especially the virus neutralizing antibodies.
    3. Mice intranasally immunized with single dose of VSV-RABVG were 100% protected upon RABV challenge.

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    1. Bommier, E., Chapat, L., Guiot, A.L., Hilaire, F., Cariou, C., Poulet, H., Pialot, D., De Luca, K., 2020. Multivariate analysis of the immune response to different rabies vaccines. Vet. Immunol. Immunopathol. 220, 109986.

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    13. Wu, C., Wu, M., Liang, M., Xiong, S., Dong, C., 2019. A novel oncolytic virus engineered with PD-L1 scFv effectively inhibits tumor growth in a mouse model. Cell. Mol. Immunol. 16, 780-782.

    14. Wu, F., Fan, X., Yue, Y., Xiong, S., Dong, C., 2014. A vesicular stomatitis virus-based mucosal vaccine promotes dendritic cell maturation and elicits preferable immune response against coxsackievirus B3 induced viral myocarditis. Vaccine 32, 3917-3926.

    15. Yahalom-Ronen, Y., Tamir, H., Melamed, S., Politi, B., Shifman, O., Achdout, H., Vitner, E.B., Israeli, O., Milrot, E., Stein, D., Cohen-Gihon, I., Lazar, S., Gutman, H., Glinert, I., Cherry, L., Vagima, Y., Lazar, S., Weiss, S., Ben-Shmuel, A., Avraham, R., Puni, R., Lupu, E., Bar-David, E., Sittner, A., Erez, N., Zichel, R., Mamroud, E., Mazor, O., Levy, H., Laskar, O., Yitzhaki, S., Shapira, S.C., Zvi, A., Beth-Din, A., Paran, N., Israely, T., 2020. A single dose of recombinant VSV-G-spike vaccine provides protection against SARS-CoV-2 challenge. Nat. Commun. 11, 6402.

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    A single dose of recombinant VSV-RABVG vaccine provides full protection against RABV challenge

      Corresponding author: Sidong Xiong, sdxiong@suda.edu.cn
      Corresponding author: Ling Zhao, zling604@yahoo.com
      Corresponding author: Chunsheng Dong, chunshengdong@suda.edu.cn
    • a Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China;
    • b State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China;
    • c Key Laboratory of Preventive Veterinary Medicine of Hubei Province, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China

    Abstract: Highlights:
    1. A replication-competent recombinant VSV with RABV-G protein replacement was generated.
    2. Single dose of VSV-RABVG immunization induce potent antigen-specific humoral immune response, especially the virus neutralizing antibodies.
    3. Mice intranasally immunized with single dose of VSV-RABVG were 100% protected upon RABV challenge.

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