摘要

关键词：
• 非洲猪瘟病毒
• , 热对流PCR
• , 现场检测

Abstract

References

1. Ambagala, A., Fisher, M., Goolia, M., Nfon, C., Furukawa-Stoffer, T., Ortega Polo, R., Lung, O., 2017. Field-deployable reverse transcription-insulated isothermal PCR (RTiiPCR) assay for rapid and sensitive detection of foot-and-mouth disease virus. Transboundary Emerg. Dis. 64, 1610-1623.
   

2. Chen, N., Ye, M., Xiao, Y., Li, S., Huang, Y., Li, X., Tian, K., Zhu, J., 2019. Development of universal and quadruplex real-time RT-PCR assays for simultaneous detection and differentiation of porcine reproductive and respiratory syndrome viruses. Transboundary Emerg. Dis. 66, 2271-2278.
   

3. Gaudreault, N.N., Richt, J.A., 2019. Subunit vaccine approaches for African swine fever virus. Vaccines 7, 56.
   

4. James, H.E., Ebert, K., McGonigle, R., Reid, S.M., Boonham, N., Tomlinson, J.A., Hutchings, G.H., Denyer, M., Oura, C.A., Dukes, J.P., King, D.P., 2010. Detection of African swine fever virus by loop-mediated isothermal amplification. J. Virol Methods 164, 68-74.
   

5. Li, X., Tian, K., 2018. African swine fever in China. Vet. Rec. 183, 300-301.
   

6. Lung, O., Pasick, J., Fisher, M., Buchanan, C., Erickson, A., Ambagala, A., 2016. Insulated isothermal reverse transcriptase PCR (iiRT-PCR) for rapid and sensitive detection of classical swine fever virus. Transboundary Emerg. Dis. 63, e395-402.
   

7. Luo, Y., Atim, S.A., Shao, L., Ayebazibwe, C., Sun, Y., Liu, Y., Ji, S., Meng, X.Y., Li, S., Li, Y., Masembe, C., Stahl, K., Widen, F., Liu, L., Qiu, H.J., 2017. Development of an updated PCR assay for detection of African swine fever virus. Arch. Virol. 162, 191-199.
   

8. Miao, F., Zhang, J., Li, N., Chen, T., Wang, L., Zhang, F., Mi, L., Wang, S., Wang, Y., Zhou, X., Zhang, Y., Li, M., Zhang, S., Hu, R., 2019. Rapid and sensitive recombinase polymerase amplification combined with lateral flow strip for detecting African swine fever virus. Front. Microbiol. 10, 1004.
   

9. Sanchez, E.G., Perez-Nunez, D., Revilla, Y., 2019. Development of vaccines against African swine fever virus. Virus Res. 265, 150-155.
   

10. Tran, H.N.T., Le, H.C.T., Pham, B.P., Luu, V.Q., Nguye, V.L., 2021. Evaluation of an automated insulated isothermal polymerase chain reaction system for rapid and reliable, on-site detection of African swine fever virus. J. Am. Vet. Med. Assoc. 15, 662-668.
    

11. Tsai, Y.L., Wang, H.T., Chang, H.F., Tsai, C.F., Lin, C.K., Teng, P.H., Su, C., Jeng, C.C., Lee, P.Y., 2012. Development of TaqMan probe-based insulated isothermal PCR (iiPCR) for sensitive and specific on-site pathogen detection. PLoS One 7, e45278.
    

12. Zhang, J., Nfon, C., Tsai, C.F., Lee, C.H., Fredericks, L., Chen, Q., Sinha, A., Bade, S., Harmon, K., Pineyro, P., Gauger, P., Tsai, Y.L., Wang, H.T., Lee, P.A., 2019. Development and evaluation of a real-time RT-PCR and a field-deployable RTinsulated isothermal PCR for the detection of Seneca Valley virus. BMC Vet. Res. 15, 168.
    

13. Zhao, D., Liu, R., Zhang, X., Li, F., Wang, J., Zhang, J., Liu, X., Wang, L., Wu, X., Guan, Y., Chen, W., Wang, X., He, X., Bu, Z., 2019. Replication and virulence in pigs of the first African swine fever virus isolated in China. Emerg. Microb. Infect. 8, 438-447.
    

14. Zhou, X., Li, N., Luo, Y., Liu, Y., Miao, F., Chen, T., Zhang, S., Cao, P., Li, X., Tian, K., Qiu, H.J., Hu, R., 2018. Emergence of African swine fever in China, 2018. Transboundary Emerg. Dis. 65, 1482-1484.
    

Proportional views

Electronic Supplementary Material

10.1016j.virs.2022.03.002-ESM.docx