. doi: 10.1016/j.virs.2022.10.005
Citation: Ruikun Du, Qinghua Cui, Zinuo Chen, Xiujuan Zhao, Xiaojing Lin, Lijun Rong. Revisiting influenza A virus life cycle from a perspective of genome balance .VIROLOGICA SINICA, 2023, 38(1) : 1-8.  http://dx.doi.org/10.1016/j.virs.2022.10.005

从基因组平衡角度重新探讨甲型流感病毒的生命周期

  • 甲型流感病毒(influenza A virus,IAV)基因组由八节段负义单链RNA组成,其复制调控机制复杂而精密,以维持动态的微平衡。本文综述了IAV基因组平衡对于病毒生命周期的重要意义,包括病毒血凝素(hemagglutin)-神经氨酸酶(Neuraminidase)的功能平衡、病毒聚合酶对于病毒RNA复制和转录的平衡调控、病毒基因组八节段复制过程中的竞争性平衡、病毒蛋白表达谱的平衡调控以及病毒包装过程中高度选择装配和随机装配的平衡。进一步,本文总结了病毒进化过程中的“平衡回归”现象,并提出病毒基因组人工修饰的“平衡补偿”策略。本综述从基因组平衡的角度重新探讨IAV的复制周期,一方面深化了对于IAV生活周期的认知,另一方面对于基础病毒学和应用病毒学研究具有重要的启发。

Revisiting influenza A virus life cycle from a perspective of genome balance

  • Influenza A virus (IAV) genome comprises eight negative-sense RNA segments, of which the replication is well orchestrated and the delicate balance of multiple segments are dynamically regulated throughout IAV life cycle. However, previous studies seldom discuss these balances except for functional hemagglutinin-neuraminidase balance that is pivotal for both virus entry and release. Therefore, we attempt to revisit IAV life cycle by highlighting the critical role of "genome balance". Moreover, we raise a "balance regression" model of IAV evolution that the virus evolves to rebalance its genome after reassortment or interspecies transmission, and direct a "balance compensation" strategy to rectify the "genome imbalance" as a result of artificial modifications during creation of recombinant IAVs. This review not only improves our understanding of IAV life cycle, but also facilitates both basic and applied research of IAV in future.

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      沈阳化工大学材料科学与工程学院 沈阳 110142

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    Revisiting influenza A virus life cycle from a perspective of genome balance

      Corresponding author: Ruikun Du, duzi857@163.com
      Corresponding author: Lijun Rong, lijun@uic.edu
    • a. Innovation Research Institute of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China;
    • b. Qingdao Academy of Chinese Medicinal Sciences, Shandong University of Traditional Chinese Medicine, Qingdao, 266122, China;
    • c. College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250355, China;
    • d. Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, Chicago, 60612, USA

    Abstract: Influenza A virus (IAV) genome comprises eight negative-sense RNA segments, of which the replication is well orchestrated and the delicate balance of multiple segments are dynamically regulated throughout IAV life cycle. However, previous studies seldom discuss these balances except for functional hemagglutinin-neuraminidase balance that is pivotal for both virus entry and release. Therefore, we attempt to revisit IAV life cycle by highlighting the critical role of "genome balance". Moreover, we raise a "balance regression" model of IAV evolution that the virus evolves to rebalance its genome after reassortment or interspecies transmission, and direct a "balance compensation" strategy to rectify the "genome imbalance" as a result of artificial modifications during creation of recombinant IAVs. This review not only improves our understanding of IAV life cycle, but also facilitates both basic and applied research of IAV in future.

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