. doi: 10.1016/j.virs.2022.12.001
Citation: Yuchang Li, Yanhong Gao, Ying Tang, Jing Li, Sen Zhang, Tao Jiang, Xiaoping Kang. Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus .VIROLOGICA SINICA, 2023, 38(1) : 165-170.  http://dx.doi.org/10.1016/j.virs.2022.12.001

基于重组酶聚合酶扩增的猴痘病毒核酸检测方法的建立

  • 猴痘病毒(MPXV)的传播引起了世界范围内的高度关注,及早、快速地发现病原体对于控制猴痘病毒感染的传播可发挥重要作用。核酸检测已成为病原鉴定的标准方法,重组聚合酶扩增(RPA)是一种在等温条件下扩增靶基因的核酸检测方法,与常用的qPCR相比,对病原体的鉴定速度更快、更简单。本研究开发了两种检测MPXV的RPA方法:荧光-RPA (F-RPA)和垂直流条带RPA (VF-RPA),F-RPA的检出限为15.32 copies/μL (95%置信区间(CI)为11.42~26.60 copies/μL),VF-RPA的检出限为8.53 copies/μL (95% CI:6.69~13.85 copies/μL),以上方法与14种发热和皮疹相关病毒无交叉反应。然后通过10个阳性样本和56个阴性样本对两种方法进行验证,结果准确。F-RPA和VF-RPA方法操作简单、快速、省时,有望用于野外/临床MPXV的检测。

Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

  • Highlights
    1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
    2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
    3. No cross-reaction was found in 14 rash and fever-associated viruses.
    4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.

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    1. Adler, H., Gould, S., Hine, P., Snell, L.B., Wong, W., Houlihan, C.F., Osborne, J.C., Rampling, T., Beadsworth, M.B., Duncan, C.J., Dunning, J., Fletcher, T.E., Hunter, E.R., Jacobs, M., Khoo, S.H., Newsholme, W., Porter, D., Porter, R.J., Ratcliffe, L., Schmid, M.L., Semple, M.G., Tunbridge, A.J., Wingfield, T., Price, N.M., NHS England High Consequence Infectious Diseases (Airborne) Network, 2022.Clinical features and management of human monkeypox:a retrospective observational study in the UK. Lancet Infect. Dis. 22, 1153-1162.

    2. Bunge, E.M., Hoet, B., Chen, L., Lienert, F., Weidenthaler, H., Baer, L.R., Steffen, R., 2022.The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Neglected Trop. Dis. 16, e0010141.

    3. Davi, S.D., Kissenkötter, J., Faye, M., Böhlken-Fascher, S., Stahl-Hennig, C., Faye, O., Faye, O., Sall, A.A., Weidmann, M., Ademowo, O.G., Hufert, F.T., Czerny, C.P., Abd El Wahed, A., 2019. Recombinase polymerase amplification assay for rapid detection of Monkeypox virus. Diagn. Microbiol. Infect. Dis. 95, 41-45.

    4. Erez, N., Achdout, H., Milrot, E., Schwartz, Y., Wiener-Well, Y., Paran, N., Politi, B., Tamir, H., Israely, T., Weiss, S., Beth-Din, A., Shifman, O., Israeli, O., Yitzhaki, S., Shapira, S.C., Melamed, S., Schwartz, E., 2019. Diagnosis of imported monkeypox, Israel, 2018. Emerg. Infect. Dis. 25, 980-983.

    5. Jia, J., Li, Y., Wu, X., Zhang, S., Hu, Y., Li, J., Jiang, T., Kang, X., 2019. Reverse transcription recombinase polymerase amplification assays for rapid detection of tick-borne encephalitis virus infection. Virol. Sin. 34, 338-341.

    6. Mauldin, M.R., McCollum, A.M., Nakazawa, Y.J., Mandra, A., Whitehouse, E.R., Davidson, W., Zhao, H., Gao, J., Li, Y., Doty, J., Yinka-Ogunleye, A., Akinpelu, A., Aruna, O., Naidoo, D., Lewandowski, K., Afrough, B., Graham, V., Aarons, E., Hewson, R., Vipond, R., Dunning, J., Chand, M., Brown, C., Cohen-Gihon, I., Erez, N., Shifman, O., Israeli, O., Sharon, M., Schwartz, E., Beth-Din, A., Zvi, A., Mak, T.M., Ng, Y.K., Cui, L., Lin, R.T.P., Olson, V.A., Brooks, T., Paran, N., Ihekweazu, C., Reynolds, M.G., 2022. Exportation of monkeypox virus from the African Continent.J. Infect. Dis. 225, 1367-1376.

    7. Miura, F., van Ewijk, C.E., Backer, J.A., Xiridou, M., Franz, E., Op de Coul, E., Brandwagt, D., van Cleef, B., van Rijckevorsel, G., Swaan, C., van den Hof, S.,Wallinga, J., 2022. Estimated incubation period for monkeypox cases confirmed in The Netherlands, May 2022. Euro Surveill. 27, 2200448.

    8. Stern, D., Olson, V.A., Smith, S.K., Pietraszczyk, M., Miller, L., Miethe, P., Dorner, B.G., Nitsche, A., 2016. Rapid and sensitive point-of-care detection of Orthopoxviruses by ABICAP immunofiltration. Virol. J. 13, 207.

    9. Tang, Y., Wang, Y., Li, Y., Zhao, H., Zhang, S., Zhang, Y., Li, J., Chen, Y., Wu, X., Qin, C., Jiang, T., Kang, X., 2022. An integrated rapid nucleic acid detection assay based on recombinant polymerase amplification for SARS-CoV-2. Virol. Sin. 37, 138-141.

    10. The International Committee on Taxonomy of Viruses (ICTV), 2022. Virus Taxonomy:2020 Release. https://talk.ictvonline.org/taxonomy.

    11. Thornhill, J.P., Barkati, S., Walmsley, S., Rockstroh, J., Antinori, A., Harrison, L.B., Palich, R., Nori, A., Reeves, I., Habibi, M.S., Apea, V., Boesecke, C., Vandekerckhove, L., Yakubovsky, M., Sendagorta, E., Blanco, J.L., Florence, E., Moschese, D., Maltez, F.M., Goorhuis, A., Pourcher, V., Migaud, P., Noe, S., Pintado, C., Maggi, F., Hansen, A.E., Hoffmann, C., Lezama, J.I., Mussini, C., Cattelan, A., Makofane, K., Tan, D., Nozza, S., Nemeth, J., Klein, M.B., Orkin, C.M., SHARE-net Clinical Group, 2022. Monkeypox virus infection in humans across 16 countries-April-June 2022. N. Engl. J. Med. 387, 679-691.

    12. Wang, S., Li, Y., Zhang, F., Jiang, N., Zhuang, Q., Hou, G., Jiang, L., Yu, J., Yu, X., Liu, H., Zhao, C., Yuan, L., Huang, B., Wang, K., 2022. Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus. Virol. J. 19, 129.

    13. World Health Organization (WHO), 2022a. Multi-country monkeypox outbreak:situation update. https://www.who.int/emergencies/disease-outbreak-news/item/2022-DON393.

    14. World Health Organization (WHO), 2022b. Surveillance, case investigation and contact tracing for monkeypox:interim guidance, 24 June 2022. https://apps.who.int/iris/handle/10665/357186.

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    Development of rapid nucleic acid assays based on the recombinant polymerase amplification for monkeypox virus

      Corresponding author: Tao Jiang, jiangtao@bmi.ac.cn
      Corresponding author: Xiaoping Kang, kangxiaoping@bmi.ac.cn
    • a. State Key Laboratory of Pathogen and Biosecurity, The Academy of Military Medical Science, Institute of Microbiology and Epidemiology, Beijing, 100071, China;
    • b. Laboratory Department of the First Medical Center, Chinese PLA General Hospital, Beijing, 100850, China

    Abstract: Highlights
    1. Two RPA methods (F-RPA and VF- RPA) have been developed for monkeypox virus rapid detection.
    2. The limit of detection was 15.32 copies/μL for F-RPA and 8.53 copies/μL for VF-RPA,
    3. No cross-reaction was found in 14 rash and fever-associated viruses.
    4. The two RPA methods developed are simple and rapid, making them potentially useful for detection of MPXV in the field/clinic.

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