. doi: 10.1016/j.virs.2023.03.006
Citation: Yuda Chen, Changcheng Wu, Ruhan A, Li Zhao, Zhongxian Zhang, Wenjie Tan. Perspective on the application of genome sequencing for monkeypox virus surveillance .VIROLOGICA SINICA, 2023, 38(2) : 327-333.  http://dx.doi.org/10.1016/j.virs.2023.03.006

基因组测序技术在猴痘病毒监测中的应用展望

  • 猴痘(Mpox)是一种病毒性人畜共患病,通常表现出皮疹、发热、头痛、肌肉疼痛等症状,严重感染可导致死亡。长期以来,猴痘主要在非洲中西部国家流行,非洲以外少数国家也报道了零星病例。2022年5月以来,多个国家相继报告了大规模猴痘病毒感染病例。当前,世界卫生组织(WHO)已将本次疫情升级为国际关注的公共卫生事件。基因组测序结果显示,引起本次疫情的毒株较已报道毒株新产生了大量突变,被证实为猴痘病毒的新进化分支。临床采集及环境监测样本中猴痘病毒基因组测序对分子溯源和病毒变异监测预警等至关重要。然而,猴痘病毒基因组较大且具有末端串联重复等复杂特征,临床采集样本类型多样且病毒载量一般较低,给基因组测序工作带来极大挑战。为了更好地进行猴痘病毒基因组测序,本文综述了该领域最新研究进展,系统地总结了猴痘病毒基因组测序的主要步骤,基于获得基因组的质量对不同测序方案进行了横向比较。此外,我们还提出了多种适用于猴痘病毒的基因组富集或扩增的优化策略。本文将为猴痘病毒基因组测序方案制定提供帮助,有利于猴痘检测、监测预警和分子溯源工作的顺利开展。

Perspective on the application of genome sequencing for monkeypox virus surveillance

  • Corresponding author: Wenjie Tan, tanwj@ivdc.chinacdc.cn
  • Received Date: 27 September 2022
    Accepted Date: 21 March 2023
  • Highlights
    1. Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity.
    2. The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained.
    3. Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated.
    4. Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.

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    Perspective on the application of genome sequencing for monkeypox virus surveillance

      Corresponding author: Wenjie Tan, tanwj@ivdc.chinacdc.cn
    • a. School of Public Health, Baotou Medical College, Baotou, 014040, China;
    • b. NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 100052, China

    Abstract: Highlights
    1. Whole genome sequencing of MPXV is crucial for monitoring emerging variants and assessing their potential pathogenicity.
    2. The critical steps of mNGS, encompassing nucleic acid extraction, library preparation, sequencing, and data analysis, are concisely explained.
    3. Optimization strategies for sample pre-processing, virus enrichment, and sequencing platform selection are deliberated.
    4. Conducting next-generation sequencing and third-generation sequencing concurrently is highly recommended.

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