Hong-Qing Zhang, Zhe-Rui Zhang, Cheng-Lin Deng, Zhi-Ming Yuan and Bo Zhang. A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation[J]. Virologica Sinica, 2024, 39(3): 516-519. doi: 10.1016/j.virs.2024.03.008
Citation: Hong-Qing Zhang, Zhe-Rui Zhang, Cheng-Lin Deng, Zhi-Ming Yuan, Bo Zhang. A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation .VIROLOGICA SINICA, 2024, 39(3) : 516-519.  http://dx.doi.org/10.1016/j.virs.2024.03.008

一种可用于EBOV靶向预防性疫苗评价的重组水泡性口炎病毒小鼠致死模型

cstr: 32224.14.j.virs.2024.03.008
  • 通讯作者: 张波, zhangbo@wh.iov.cn
  • 收稿日期: 2023-10-09
    录用日期: 2024-03-18
  • 埃博拉病毒(Ebola virus,EBOV)具有高度致病性,能在人类和非人灵长类动物(NHPs)中引发严重的埃博拉病毒病,并伴随着高死亡率。由于其极端的危险性,对EBOV的相关研究只能在生物安全级4级实验室(Biosafety level 4, BSL-4)中进行。此外,研究人员还需具备高超的实验技能和深厚的专业知识,以确保研究的安全性和准确性。这些因素共同限制了EBOV研究的进展。因此,建立一种能用于评估EBOV疫苗候选株的替代动物模型,具有十分重要的意义。近几年,一种表面整合有EBOV糖蛋白(GP)的重组水泡性口炎病毒(VSV-EBOV) 已获批上市,作为减毒活疫苗,它具有良好的安全性。本文介绍了一种基于VSV-EBOV感染的IFNα/β/γ R-/-小鼠致死模型。IFNα/β/γ R-/-小鼠对VSV-EBOV高度易感,感染后表现为高水平的病毒血症、广泛的病毒组织分布、明显的肝脾病变以及高致死性。最重要的是,通过两次腹腔接种EBOV VRPs(一种EBOV疫苗候选株),可诱导小鼠产生较为可观的中和抗体,并成功保护IFNα/β/γ R-/-小鼠免受VSV-EBOV的致死性感染。综上所述,基于VSV-EBOV感染的IFNα/β/γ R-/-小鼠可作为一种体内评价EBOV预防性疫苗的平台。

A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation

  • Corresponding author: Bo Zhang, zhangbo@wh.iov.cn
  • Received Date: 09 October 2023
    Accepted Date: 18 March 2024
  • Highlights1. The recombinant VSV-EBOV is highly lethal to IFNα/β/γ R-/- mice.2. The infected mice exhibit hyperviremia and distinct splenohepatic lesions.3. Virus replicon particle vaccine can protect all IFNα/β/γ R-/- mice against VSV-EBOV lethal challenge.4. This surrogate model can be used for EBOV vaccine evaluation.

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    A lethal mice model of recombinant vesicular stomatitis viruses for EBOV-targeting prophylactic vaccines evaluation

      Corresponding author: Bo Zhang, zhangbo@wh.iov.cn
    • a. Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China;
    • b. University of Chinese Academy of Sciences, Beijing, 100049, China;
    • c. Hubei Jiangxia Laboratory, Wuhan, 430200, China

    Abstract: Highlights1. The recombinant VSV-EBOV is highly lethal to IFNα/β/γ R-/- mice.2. The infected mice exhibit hyperviremia and distinct splenohepatic lesions.3. Virus replicon particle vaccine can protect all IFNα/β/γ R-/- mice against VSV-EBOV lethal challenge.4. This surrogate model can be used for EBOV vaccine evaluation.

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