Shengyao Chen, Muhammad Saqib, Hafiz Sajid Khan, Yuan Bai, Usman Ali Ashfaq, Muhammad Khalid Mansoor, Abulimti Moming, Jing Liu, Min Zhou, Saifullah Khan Niazi, Qiaoli Wu, Awais-Ur-Rahman Sial, Shuang Tang, Muhammad Hassan Sarfraz, Aneela Javed, Sumreen Hayat, Mohsin Khurshid, Iahtasham Khan, Muhammad Ammar Athar, Zeeshan Taj, Bo Zhang, Fei Deng, Ali Zohaib and Shu Shen. Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence[J]. Virologica Sinica, 2024, 39(3): 369-377. doi: 10.1016/j.virs.2024.04.001
Citation: Shengyao Chen, Muhammad Saqib, Hafiz Sajid Khan, Yuan Bai, Usman Ali Ashfaq, Muhammad Khalid Mansoor, Abulimti Moming, Jing Liu, Min Zhou, Saifullah Khan Niazi, Qiaoli Wu, Awais-Ur-Rahman Sial, Shuang Tang, Muhammad Hassan Sarfraz, Aneela Javed, Sumreen Hayat, Mohsin Khurshid, Iahtasham Khan, Muhammad Ammar Athar, Zeeshan Taj, Bo Zhang, Fei Deng, Ali Zohaib, Shu Shen. Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence .VIROLOGICA SINICA, 2024, 39(3) : 369-377.  http://dx.doi.org/10.1016/j.virs.2024.04.001

基于血清流行的巴基斯坦健康人群感染虫媒病毒的风险

  • 虫媒病毒引起的传染病是巴基斯坦的公共卫生问题。然而,关于虫媒病毒的数据流行率和导致威胁的研究是有限的。本研究基于新建立的荧光素酶免疫沉淀系统(LIPS)调查了巴基斯坦健康人群中虫媒病毒的血清流行率,包括严重发热伴血小板减少综合征病毒(SFTSV)、克里米亚-刚果出血热病毒(CCHFV)、Tamdy virus(TAMV)和karshi virus(KSIV),以及寨卡病毒(ZIKV)的酶联免疫吸附测定(ELISA)。然后从抗体阳性样品中进一步检测对这些虫媒病毒的中和活性。研究结果显示:巴基斯坦健康人群中SFTSV、CCHFV、TAMV、KSIV和ZIKV的血清流行率分别为17.37%、7.58%、4.41%、1.10%和6.48%,对SFTSV、CCHFV和ZIKV的中和抗体阳性率分别为1.79%、2.62%、0.69%;此外,在SFTSV抗体阳性血清中还检测到对SFTSV相关的Guertu病毒的中和活性,比率为0.83%。研究结果还证明了存在同一个体对多种虫媒病毒血清暴露的现象,发现37人具有多种病毒的抗体,其中13人表现出中和活性,提示巴基斯坦多种虫媒病毒的溢出风险具有复杂性。分析了与暴露发生率和抗体反应水平相关的风险因素,男性、年龄≤40岁的个体以及户外工作者暴露于虫媒病毒的风险相对高。本研究揭示了巴基斯坦人群对多种虫媒病毒感染暴露的风险,提示巴基斯坦虫媒病毒流行的复杂性,有必要扩大虫媒病毒流行调查的地区和人群,为改善巴基斯坦健康监测提供重要基础数据。

Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

  • Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo hemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi virus (KSIV) based on a newly established luciferase immunoprecipitation system (LIPS) assays, and Zika virus (ZIKV) by enzyme-linked immunosorbent assays (ELISA). Neutralizing activities against these arboviruses were further examined from the antibody positive samples. The results showed that the seroprevalence of SFTSV, CCHFV, TAMV, KSIV, and ZIKV was 17.37%, 7.58%, 4.41%, 1.10%, and 6.48%, respectively, and neutralizing to SFTSV (1.79%), CCHFV (2.62%), and ZIKV (0.69%) were identified, as well as to the SFTSV-related Guertu virus (GTV, 0.83%). Risk factors associated with the incidence of exposure and levels of antibody response were analyzed. Moreover, co-exposure to different arboviruses was demonstrated, as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing activity. Males, individuals aged ≤40 years, and outdoor workers had a high risk of exposure to arboviruses. All these results reveal the substantial risks of infection with arboviruses in Pakistan, and indicate the threat from co-exposure to multiple arboviruses. The findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

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    Risk of infection with arboviruses in a healthy population in Pakistan based on seroprevalence

      Corresponding author: Fei Deng, df@wh.iov.cn
      Corresponding author: Ali Zohaib, ali.zohaib@iub.edu.pk
      Corresponding author: Shu Shen, shenshu@wh.iov.cn
    • a. Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China;
    • b. University of Chinese Academy of Sciences, Beijing 100049, China;
    • c. Faculty of Veterinary Science, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan;
    • d. The Children's Hospital Faisalabad, 38000, Pakistan;
    • e. Department of Bioinformatics and Biotechnology, Government College University Faisalabad, Faisalabad 38000, Pakistan;
    • f. Department of Microbiology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan;
    • g. Hubei Jiangxia Laboratory, Wuhan 430200, China;
    • h. Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China;
    • i. Armed Forces Institute of Pathology (AFIP), Rawalpindi 46000, Pakistan;
    • j. Department of Clinical Studies, Faculty of Veterinary & Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan;
    • k. Institute of Microbiology, Government College University Faisalabad, Faisalabad 38000, Pakistan;
    • l. Atta-ur-Rahman School of Applied Biosciences, National University of Science and Technology, Islamabad 44000, Pakistan;
    • m. Department of Clinical Sciences, University of Veterinary and Animal Sciences, Lahore Sub-campus, Jhang 35200, Pakistan;
    • n. Department of Molecular Pathology, National Medical Center DHA Phase 1, Karachi 75500, Pakistan

    Abstract: Infectious diseases caused by arboviruses are a public health concern in Pakistan. However, studies on data prevalence and threats posed by arboviruses are limited. This study investigated the seroprevalence of arboviruses in a healthy population in Pakistan, including severe fever with thrombocytopenia syndrome virus (SFTSV), Crimean-Congo hemorrhagic fever virus (CCHFV), Tamdy virus (TAMV), and Karshi virus (KSIV) based on a newly established luciferase immunoprecipitation system (LIPS) assays, and Zika virus (ZIKV) by enzyme-linked immunosorbent assays (ELISA). Neutralizing activities against these arboviruses were further examined from the antibody positive samples. The results showed that the seroprevalence of SFTSV, CCHFV, TAMV, KSIV, and ZIKV was 17.37%, 7.58%, 4.41%, 1.10%, and 6.48%, respectively, and neutralizing to SFTSV (1.79%), CCHFV (2.62%), and ZIKV (0.69%) were identified, as well as to the SFTSV-related Guertu virus (GTV, 0.83%). Risk factors associated with the incidence of exposure and levels of antibody response were analyzed. Moreover, co-exposure to different arboviruses was demonstrated, as thirty-seven individuals were having antibodies against multiple viruses and thirteen showed neutralizing activity. Males, individuals aged ≤40 years, and outdoor workers had a high risk of exposure to arboviruses. All these results reveal the substantial risks of infection with arboviruses in Pakistan, and indicate the threat from co-exposure to multiple arboviruses. The findings raise the need for further epidemiologic investigation in expanded regions and populations and the necessity to improve health surveillance in Pakistan.

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