Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai, China, 2022

Jiayu Wang; Wencheng Wu; Wei Wang; Jingyi Zhang; Jianwei Xiao; Mingyi Cai; Jiaying Guo; Run Li; Shuangli Zhu; Wanju Zhang; Min Chen; Zheng Teng

doi:10.1016/j.virs.2024.05.008

August 2024

Jiayu Wang, Wencheng Wu, Wei Wang, Jingyi Zhang, Jianwei Xiao, Mingyi Cai, Jiaying Guo, Run Li, Shuangli Zhu, Wanju Zhang, Min Chen and Zheng Teng. Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai, China, 2022[J]. Virologica Sinica, 2024, 39(4): 694-698. doi: 10.1016/j.virs.2024.05.008

Citation: Jiayu Wang, Wencheng Wu, Wei Wang, Jingyi Zhang, Jianwei Xiao, Mingyi Cai, Jiaying Guo, Run Li, Shuangli Zhu, Wanju Zhang, Min Chen, Zheng Teng. Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai, China, 2022 .VIROLOGICA SINICA, 2024, 39(4) : 694-698. http://dx.doi.org/10.1016/j.virs.2024.05.008

2022年上海地区健康人群中手足口病相关肠道病毒血清中和抗体阳性率调查

王嘉瑜 ^a ,
吴文成 ^a ,
王葳 ^a ,
张靖翊 ^a ,
肖建伟 ^b ,
蔡明毅 ^c ,
郭家胤 ^d ,
李润 ^a ,
祝双利 ^e ,
张万菊 ^a ,
陈敏 ^a ,
滕峥 ^a,,

cstr: 32224.14.j.virs.2024.05.008

a. 上海市疾病预防控制中心病原生物检定所;
b. 上海市杨浦区疾病预防控制中心微生物实验室;
c. 上海市静安区疾病预防控制中心微生物检验科;
d. 上海市长宁区疾病预防控制中心微生物检验科;
e. 中国疾病预防控制中心病毒病预防控制所

通讯作者： 滕峥, tengzheng@scdc.sh.cn
收稿日期： 2023-10-20
录用日期： 2024-05-20

摘要

肠道病毒A71型（EV-A71）、柯萨奇病毒A16型（CVA16）、柯萨奇病毒A6型（CVA6）、柯萨奇病毒A10型（CVA10）和柯萨奇病毒A4型（CVA4）是引起手足口病的主要病原体。本研究采集了307名年龄在3天至69岁之间的健康人血清标本，调查2022年上海地区5种手足口病相关肠道病毒血清中和抗体阳性率及滴度。研究结果表明，5种肠道病毒在健康人群中血清阳性率在40.07%-67.43%之间，其中CVA16中和抗体血清阳性率最低。柯萨奇病毒血清中和抗体阳性率在不同年龄组人群中与EV-A71呈现截然不同的分布趋势。在5岁以下儿童群体中，柯萨奇病毒保护性中和抗体血清阳性率较低，这提示在手足口病易感人群中，肠道病毒传播及流行的风险较高。此外，本研究也证实了CVA4在老年人群体中的隐性传播和流行。综上所述，这是2019年新冠病毒疫情后上海市首次报道手足口病相关肠道病毒血清流行病学特征。我们的数据可能有助于反应在健康人群中肠道病毒的感染史及群体免疫状况，为手足口病的防控提供一定科学依据。
- 手足口病
- , 血清阳性率
- , 中和抗体
- , 肠道病毒

Seroprevalence of neutralizing antibodies against HFMD associated enteroviruses among healthy individuals in Shanghai, China, 2022

Jiayu Wang ^a ,
Wencheng Wu ^a ,
Wei Wang ^a ,
Jingyi Zhang ^a ,
Jianwei Xiao ^b ,
Mingyi Cai ^c ,
Jiaying Guo ^d ,
Run Li ^a ,
Shuangli Zhu ^e ,
Wanju Zhang ^a ,
Min Chen ^a ,
Zheng Teng ^a,,

a. Microbiology Department, Shanghai Municipal Center for Disease Control and Prevention, Shanghai, 200336, China;
b. Microbiology Department, Yangpu Municipal Center for Disease Control and Prevention, Shanghai, 200090, China;
c. Microbiology Department, Jing’an Municipal Center for Disease Control and Prevention, Shanghai, 200070, China;
d. Microbiology Department, Changning Municipal Center for Disease Control and Prevention, Shanghai, 200050, China;
e. WHO WPRO Regional Polio Reference Laboratory, NHC Key Laboratory for Biosafety, NHC Key Laboratory for Medical Virology, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China

Corresponding author: Zheng Teng, tengzheng@scdc.sh.cn
Received Date: 20 October 2023
Accepted Date: 20 May 2024

Abstract

Highlights1. The first report of serological characteristics of HFMD associated enteroviruses in Shanghai after the COVID-19 epidemic.2. The seroprevalence of protective NtAbs against Coxsackieviruses was low within the susceptible population of HFMD.3. The presence of a silent outbreak and circulation of CVA4 was confirmed among elderly individuals.

References
1. Gao, F., Bian, L.L., Chen, L., Zhou, Y.P., Li, G.F., Mao, Q.Y., He, Q., Wu, X., Yao, S.S., Yang, X.M.,Liang, Z.L., 2021. A cross-sectional seroepidemiology study of seven major enteroviruses causing HFMD in Guangdong, China. J Infect, 83, 119-145.
2. Geng, Y., Zhang, L., 2022. Impact of non-pharmaceutical interventions during COVID-19 pandemic on pertussis, scarlet fever and hand-foot-mouth disease in China. J Infect, 84, e13-e15.
3. Huang, W.C., Huang, L.M., Kao, C.L., Lu, C.Y., Shao, P.L., Cheng, A.L., Fan, T.Y., Chi, H.,Chang, L.Y., 2012. Seroprevalence of enterovirus 71 and no evidence of crossprotection of enterovirus 71 antibody against the other enteroviruses in kindergarten children in Taipei city. J Microbiol Immunol Infect, 45, 96-101.
4. Li, P., Chen, Y., Tang, A., Gao, F., Yan, J.B., 2021. Seroprevalence of coxsackievirus A16 antibody among people of various age groups: a systematic review and meta-analysis. Arch Public Health, 79, 166.
5. Shen, L., Sun, M., Song, S., Hu, Q., Wang, N., Ou, G., Guo, Z., Du, J., Shao, Z., Bai, Y., Liu, K., 2022. The impact of anti-COVID-19 nonpharmaceutical interventions on hand, foot, and mouth disease-A spatiotemporal perspective in Xi'an, northwestern China. J Med Virol, 94, 3121-3132.
6. Song D., 2020. Serological Survey of Hand, Foot and Mouth Disease among Healthy People in Hebei District, Tianjin City, 2016-2019 Year. Medical Information, 33, 147-149. (In Chinese).
7. Wang, J., Liu, J., Fang, F., Wu, J., Ji, T., Yang, Y., Liu, L., Li, C., Zhang, W., Zhang, X., Teng, Z., 2022. Genomic surveillance of coxsackievirus A10 reveals genetic features and recent appearance of genogroup D in Shanghai, China, 2016-2020. Virol Sin, 37, 177-186.
8. Wang, J., Teng, Z., Cui, X., Li, C., Pan, H., Zheng, Y., Mao, S., Yang, Y., Wu, L., Guo, X., Zhang, X., Zhu, Y., 2018. Epidemiological and serological surveillance of hand-foot-and-mouth disease in Shanghai, China, 2012-2016. Emerg Microbes Infect, 7, 8.
9. Wang, J.X., Zhu, S.L., Wang, J., Lin, Y., Pei, Y.W., Sun, D.P., Zhang, Y., Wang, X.J., Xu, W.B., Ding, S.J., 2016. Seroprevalence of Enterovirus A71 and Coxsackievirus A16 in Healthy People in Shandong Province, China. PLoS One, 11, e0162373.
10. Yang, B., Liu, F., Liao, Q., Wu, P., Chang, Z., Huang, J., Long, L., Luo, L., Li, Y., Leung, G.M., Cowling, B.J., Yu, H., 2017. Epidemiology of hand, foot and mouth disease in China, 2008 to 2015 prior to the introduction of EV-A71 vaccine. Euro Surveill, 22.
11. Zhou, Y., Van Tan, L., Luo, K., Liao, Q., Wang, L., Qiu, Q., Zou, G., Liu, P., Anh, N.T., Hong, N.T.T., He, M., Wei, X., Yu, S., Lam, T.T., Cui, J., Van Doorn, H.R.,Yu, H., 2021. Genetic Variation of Multiple Serotypes of Enteroviruses Associated with Hand, Foot and Mouth Disease in Southern China. Virol Sin, 36, 61-74.
12. Zhou, Y., Zhou, J., Yang, J., Qiu, Q., Wang, L., Yang, J., Li, Y., Liang, L., Cui, P., Cheng, Y., Zheng, W., Shi, H., Gong, H., Wang, K., Zhou, C., Chu, J.J.H., Yu, H., 2022. Comparison of Neutralizing Antibody Response Kinetics in Patients with Hand, Foot, and Mouth Disease Caused by Coxsackievirus A16 or Enterovirus A71: A Longitudinal Cohort Study of Chinese Children, 2017-2019. J Immunol, 209, 280-287.
13. Zhu, F.C., Meng, F.Y., Li, J.X., Li, X.L., Mao, Q.Y., Tao, H., Zhang, Y.T., Yao, X., Chu, K., Chen, Q.H., Hu, Y.M., Wu, X., Liu, P., Zhu, L.Y., Gao, F., Jin, H., Chen, Y.J., Dong, Y.Y., Liang, Y.C., Shi, N.M., Ge, H.M., Liu, L., Chen, S.G., Ai, X., Zhang, Z.Y., Ji, Y.G., Luo, F.J., Chen, X.Q., Zhang, Y., Zhu, L.W., Liang, Z.L., Shen, X.L., 2013. Efficacy, safety, and immunology of an inactivated alum-adjuvant enterovirus 71 vaccine in children in China: a multicentre, randomised, double-blind, placebo-controlled, phase 3 trial. Lancet, 381, 2024-2032.
14. Zhu, R., Cheng, T., Yin, Z., Liu, D., Xu, L., Li, Y., Wang, W., Liu, J., Que, Y., Ye, X., Tang, Q., Zhao, Q., Ge, S., He, S., Xia, N., 2018. Serological survey of neutralizing antibodies to eight major enteroviruses among healthy population. Emerg Microbes Infect, 7, 2.
Proportional views
Electronic Supplementary Material

10.1016j.virs.2024.05.008-ESM.docx