Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice

An Wang; Xiao-Xu Zhu; Yuanyuan Bie; Bowen Zhang; Wenting Ji; Jing Lou; Muhan Huang; Xi Zhou; Yujie Ren

doi:10.1016/j.virs.2024.08.006

October 2024

. doi: 10.1016/j.virs.2024.08.006

Citation: An Wang, Xiao-Xu Zhu, Yuanyuan Bie, Bowen Zhang, Wenting Ji, Jing Lou, Muhan Huang, Xi Zhou, Yujie Ren. Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice .VIROLOGICA SINICA, 2024, 39(5) : 782-792. http://dx.doi.org/10.1016/j.virs.2024.08.006

单细胞RNA测序揭示人巨细胞病毒感染的人源化小鼠的免疫细胞反应

王岸 ^a,b,c ,
朱晓旭 ^a,b,c ,
别渊源 ^a,b,c ,
张博文 ^a,b,c ,
姬雯婷 ^a,b,d ,
娄静 ^a,b ,
黄霂晗 ^a,b ,
周溪 ^a,b,c,d,, ,
任玉洁 ^a,b,c,,

cstr: 32224.14.j.virs.2024.08.006

a. 中国科学院武汉病毒研究所高致病性病毒与生物安全重点实验室, 武汉;
b. 中国科学院武汉病毒研究所病毒学国家重点实验室, 武汉;
c. 中国科学院大学, 北京;
d. 中国科学技术大学生命科学与医学部生命科学学院, 合肥

通讯作者： 周溪, zhouxi@wh.iov.cn; 任玉洁, renyujie@wh.iov.cn
收稿日期： 2024-04-29
录用日期： 2024-08-12

摘要

人类巨细胞病毒（HCMV）是一种常见的疱疹病毒，持续感染着世界上大部分人口。尽管宿主免疫反应强大，但HCMV仍能复制、逃避宿主防御，并通过多种免疫调节策略在宿主细胞建立潜伏感染，因此研究HCMV感染与宿主反应之间的相互作用尤为重要。HCMV具有严格的宿主特异性，特异性感染人类。因此，大多数HCMV的体内研究依赖于临床样本。幸运的是，人源化小鼠模型的建立使得实验室内进行HCMV感染动物实验变得方便。单细胞RNA测序使我们能够在宿主单细胞水平上研究病毒与宿主基因表达之间的关系。在这项研究中，我们评估了HCMV感染的人源化小鼠中PBMCs的单细胞基因表达变化，为研究HCMV感染下的病毒-宿主相互作用提供了有价值的数据集。
- 人巨细胞病毒（HCMV）
- , 单细胞RNA测序
- , PBMC
- , 病毒感染

Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice

An Wang ^a,b,c ,
Xiao-Xu Zhu ^a,b,c ,
Yuanyuan Bie ^a,b,c ,
Bowen Zhang ^a,b,c ,
Wenting Ji ^a,b,d ,
Jing Lou ^a,b ,
Muhan Huang ^a,b ,
Xi Zhou ^a,b,c,d,, ,
Yujie Ren ^a,b,c,,

a. Key Laboratory of Virology and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
b. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China;
c. University of Chinese Academy of Sciences, Beijing, 100049, China;
d. School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China

Corresponding author: Xi Zhou, zhouxi@wh.iov.cn Yujie Ren, renyujie@wh.iov.cn
Received Date: 29 April 2024
Accepted Date: 12 August 2024

Abstract

Human cytomegalovirus (HCMV) is a common herpesvirus that persistently infects a large portion of the world's population. Despite the robust host immune response, HCMV is able to replicate, evade host defenses, and establish latency throughout the lifespan by developing multiple immunomodulatory strategies, making the studies on the interaction between HCMV infection and host response particularly important. HCMV has a strict host specificity that specifically infects humans. Therefore, most of the in vivo researches of HCMV rely on clinical samples. Fortunately, the establishment of humanized mouse models allows for convenient in-lab animal experiments involving HCMV infection. Single-cell RNA sequencing enables the study of the relationship between viral and host gene expressions at the single-cell level within host cells. In this study, we assessed the gene expression alterations of PBMCs at the single-cell level within HCMV-infected humanized mice, which sheds light onto the virus-host interactions in the context of HCMV infection of humanized mice and provides a valuable dataset for the related researches.
- Human cytomegalovirus (HCMV)
- , Single-cell RNA-Sequencing
- , PBMC
- , Viral infection

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Electronic Supplementary Material

10.1016j.virs.2024.08.006-ESM.docx