. doi: 10.1016/j.virs.2024.09.009
Citation: Pengfei Hao, Zhaoxia Pang, Qiaoqiao Qu, Chunmei Cui, Yuhang Jiang, Jing Chen, Zihan Gao, Zhiqiang Xu, Letian Li, Ningyi Jin, Chang Li. A G3P[3] bat rotavirus can infect cultured human cholangiocytes and cause biliary atresia symptom in suckling mice .VIROLOGICA SINICA, 2024, 39(6) : 974-976.  http://dx.doi.org/10.1016/j.virs.2024.09.009

一株G3P[3]型蝙蝠轮状病毒可以感染体外培养的人胆管细胞以及可以引起乳鼠胆道闭锁症状

cstr: 32224.14.j.virs.2024.09.009
  • 轮状病毒是一种重要的人兽共患病毒,腹泻是其常见症状,此外,轮状病毒也被认为是胆道闭锁的诱因之一。此前已有包括RRV株(G3P[3]型)在内的数个轮状病毒毒株被证明可导致小鼠产生胆道闭锁症状,并且其VP4上的SRL肽段被证明在其中发挥重要作用。MSLH14株轮状病毒为A群G3P[3]型轮状病毒,分离于蝙蝠,并且此前已被证明可导致昆明鼠产生严重腹泻。由于该毒株与RRV同属于G3P[3]型轮状病毒,并且包含SRL肽段,于是本研究进一步探索该毒株是否可以导致小鼠产生胆道闭锁症状。研究结果表明,经腹腔注射,该毒株可导致BALB/c乳鼠出现胆道闭锁症状,MMP7、GGT、ALT、AST等相关关键指标显著升高,此外,本研究也发现,MSLH14可以体外感染人胆管细胞,表明了潜在的公共卫生风险。

A G3P[3] bat rotavirus can infect cultured human cholangiocytes and cause biliary atresia symptom in suckling mice

  • Highlights
    1 Bat rotavirus strain MSLH14 (G3P[3]) can infect human cholangiocytes in vitro.
    2 MSLH14 can cause suckling mice biliary atresia symptom, indicating potential public health risks.
    3 MSLH14 have the potential for establishing a biliary atresia animal model.

  • 加载中
    1. Averbukh, L.D., Wu, G.Y., 2018. Evidence for viral induction of biliary atresia: a review. J Clin Transl Hepatol, 6, 410-419.

    2. He, B., Huang, X., Zhang, F., Tan, W., Matthijnssens, J., Qin, S., Xu, L., Zhao, Z., Yang, L., Wang, Q., Hu, T., Bao, X., Wu, J., Tu, C., 2017. Group A rotaviruses in Chinese bats: genetic composition, serology, and evidence for bat-to-human transmission and reassortment. J. Virol., 91, e02493-16.

    3. He, B., Yang, F., Yang, W., Zhang, Y., Feng, Y., Zhou, J., Xie, J., Feng, Y., Bao, X., Guo, H., Li, Y., Xia, L., Li, N., Matthijnssens, J., Zhang, H., Tu, C., 2013. Characterization of a novel G3P[3] rotavirus isolated from a lesser horseshoe bat: a distant relative of feline/canine rotaviruses. J. Virol., 87, 12357-12366.

    4. Jiang, J., Wang, J., Shen, Z., Lu, X., Chen, G., Huang, Y., Dong, R., Zheng, S., 2019. Serum MMP-7 in the diagnosis of biliary atresia. Pediatrics, 144, e20190902.

    5. Lertudomphonwanit, C., Mourya, R., Fei, L., Zhang, Y., Gutta, S., Yang, L., Bove, K.E., Shivakumar, P., Bezerra, J.A., 2017. Large-scale proteomics identifies MMP-7 as a sentinel of epithelial injury and of biliary atresia. Sci. Transl. Med., 9, eaan8462.

    6. Meng, F., Francis, H., Glaser, S., Han, Y., Demorrow, S., Stokes, A., Staloch, D., Venter, J., White, M., Ueno, Y., Reid, L.M., Alpini, G., 2012. Role of stem cell factor and granulocyte colony-stimulating factor in remodeling during liver regeneration. Hepatology, 55, 209-221.

    7. Mohanty, S.K., Donnelly, B., Lobeck, I., Walther, A., Dupree, P., Coots, A., Meller, J., Mcneal, M., Sestak, K., Tiao, G., 2017. The SRL peptide of rhesus rotavirus VP4 protein governs cholangiocyte infection and the murine model of biliary atresia. Hepatology, 65, 1278-1292.

    8. Mohanty, S.K., Lobeck, I., Donnelly, B., Dupree, P., Walther, A., Mowery, S., Coots, A., Bondoc, A., Sheridan, R.M., Poling, H.M., Temple, H., Mcneal, M., Sestak, K., Bansal, R., Tiao, G., 2020. Rotavirus reassortant-induced murine model of liver fibrosis parallels human biliary atresia. Hepatology, 71, 1316-1330.

    9. Thomas, H., 2018. Biliary tract: MMP7-a diagnostic biomarker for biliary atresia. Nat. Rev. Gastroenterol. Hepatol., 15, 68.

    10. Wang, J., Xu, Y., Chen, Z., Liang, J., Lin, Z., Liang, H., Xu, Y., Wu, Q., Guo, X., Nie, J., Lu, B., Huang, B., Xian, H., Wang, X., Wu, Q., Zeng, J., Chai, C., Zhang, M., Lin, Y., Zhang, L., Zhao, S., Tong, Y., Zeng, L., Gu, X., Chen, Z.G., Yi, S., Zhang, T., Delfouneso, D., Zhang, Y., Nutt, S.L., Lew, A.M., Lu, L., Bai, F., Xia, H., Wen, Z., Zhang, Y., 2020. Liver immune profiling reveals pathogenesis and therapeutics for biliary atresia. Cell, 183, 1867-1883.e26.

    11. Xia, L.L., He, B., Hu, T.S., Zhang, W.D., Wang, Y.Y., Xu, L., Li, N., Qiu, W., Yu, J., Fan, Q.S., Zhang, F.Q., Tu, C.C., 2013. Isolation and characterization of rotavirus from bat. Bingdu Xuebao, 29, 632-637. (In Chinese).

    12. Yang, L., Zhou, Y., Xu, P.P., Mourya, R., Lei, H.Y., Cao, G.Q., Xiong, X.L., Xu, H., Duan, X.F., Wang, N., Fei, L., Chang, X.P., Zhang, X., Jiang, M., Bezerra, J.A., Tang, S.T., 2018. Diagnostic accuracy of serum matrix metalloproteinase-7 for biliary atresia. Hepatology, 68, 2069-2077.

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  • 10.1016j.virs.2024.09.009-ESM.docx

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    A G3P[3] bat rotavirus can infect cultured human cholangiocytes and cause biliary atresia symptom in suckling mice

      Corresponding author: Letian Li, letian823@163.com
      Corresponding author: Ningyi Jin, ningyik@126.com
      Corresponding author: Chang Li, lichang78@163.com
    • a. State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, 130062, China;
    • b. Research Unit of Key Technologies for Prevention and Control of Virus Zoonoses, Chinese Academy of Medical Sciences, Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, 130122, China

    Abstract: Highlights
    1 Bat rotavirus strain MSLH14 (G3P[3]) can infect human cholangiocytes in vitro.
    2 MSLH14 can cause suckling mice biliary atresia symptom, indicating potential public health risks.
    3 MSLH14 have the potential for establishing a biliary atresia animal model.

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