Generation and characterization of a novel ovariole cell line derived from <em>Spodoptera frugiperda</em> in China with sensitivity to both SfMNPV and AcMNPV

Yan Tong; Wenyi Jin; Xuan Li; Lin Guo; Gang Luo; Qian Meng; Jihong Zhang; Qilian Qin; Huan Zhang

doi:10.1016/j.virs.2024.10.002

December 2024

. doi: 10.1016/j.virs.2024.10.002

Citation: Yan Tong, Wenyi Jin, Xuan Li, Lin Guo, Gang Luo, Qian Meng, Jihong Zhang, Qilian Qin, Huan Zhang. Generation and characterization of a novel ovariole cell line derived from Spodoptera frugiperda in China with sensitivity to both SfMNPV and AcMNPV .VIROLOGICA SINICA, 2024, 39(6) : 929-937. http://dx.doi.org/10.1016/j.virs.2024.10.002

对SfMNPV和AcMNPV均敏感的中国草地贪夜蛾卵巢管细胞系的建立和特征

佟岩 ^a,c ,
靳雯怡 ^a,c ,
李瑄 ^a ,
郭琳 ^a,c ,
罗刚 ^b ,
孟茜 ^a ,
张继红 ^a ,
秦启联 ^a ,
张寰 ^a,,

cstr: 32224.14.j.virs.2024.10.002

a. 中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室;
b. 云南宁洱哈尼族彝族自治县植保植检站;
c. 中国科学院大学

通讯作者： 张寰, zhanghuan@ioz.ac.cn
收稿日期： 2024-01-27
录用日期： 2024-10-09

摘要

草地贪夜蛾核多角体病毒( SfMNPV )属于α-杆状病毒属，近期被我国登记为新型病毒生物杀虫剂。该病毒对2019年以来在中国云南省发现的全球性重大农业害虫草地贪夜蛾具有专化作用。为了深入了解病毒感染、复制过程和病毒颗粒的复杂形成机制，细胞系是体外研究必不可少的工具。草地贪夜蛾IPLB - Sf9和IPLB - Sf21细胞系常用于研究苜蓿银纹夜蛾核型多角体病毒( AcMNPV )的感染和复制机制，但其在SfMNPV感染后产生病毒多角体的能力并不理想。本研究从中国云南草地贪夜蛾种群中建立了一个新的细胞系IOZCAS-Sf-1。线粒体COX1基因分析验证了IOZCAS-Sf-1细胞系的种属来源。进一步对IOZCAS-Sf-1与IPLB-Sf9细胞系的COX1基因序列进行比较分析，证实了两者之间的差异。此外，IOZCAS-Sf-1细胞的另一个特性是在感染AcMNPV和SfMNPV后均具有产生多角体的显著能力。这株细胞系不仅能用于研究病毒复制的分子机制及其对宿主细胞的影响，也可以用于探究SfMNPV DNA的转染效率，是极具价值的生物资源。本研究为推进更有效的生物农药和可持续农业实践奠定了理论基础。
- 草地贪夜蛾核型多角体病毒
- , 苜蓿银纹夜蛾核型多角体病毒
- , 草地贪夜蛾
- , 线粒体COX1基因
- , 昆虫卵巢细胞系

Generation and characterization of a novel ovariole cell line derived from Spodoptera frugiperda in China with sensitivity to both SfMNPV and AcMNPV

Yan Tong ^a,c ,
Wenyi Jin ^a,c ,
Xuan Li ^a ,
Lin Guo ^a,c ,
Gang Luo ^b ,
Qian Meng ^a ,
Jihong Zhang ^a ,
Qilian Qin ^a ,
Huan Zhang ^a,,

a. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China;
b. Yunnan Ning'er Hani and Yi Autonomous County Plant Protection Plant Inspection Station, Ning'er, 665199, China;
c. University of Chinese Academy of Sciences, Beijing, 101408, China

Corresponding author: Huan Zhang, zhanghuan@ioz.ac.cn
Received Date: 27 January 2024
Accepted Date: 09 October 2024

Abstract

Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), belonging to the species Alphabaculovirus spofrugiperdae, has been recently registered as an insecticide in China. This virus has a specific effect on the global major agricultural pest Spodoptera frugiperda. To gain insights into viral infection, replication processes, and the complex formation of viral particles, in vitro studies using cell lines are essential tools. Although the IPLB-Sf9 and IPLB-Sf21 cell lines derived from S. frugiperda are widely used for studies on the infection and replication mechanisms of Autographa californica multiple nucleopolyhedrovirus (AcMNPV), their capacity to produce viral polyhedra after SfMNPV infection is not optimal. To address this limitation, a novel cell line named IOZCAS-Sf-1 was developed from a S. frugiperda population in Yunnan, China. The mitochondrial COX1 gene analysis confirmed the species origin of the IOZCAS-Sf-1 cell line. Furthermore, a comparative study was carried out to contrast the COX1 gene sequence of this novel cell line with that of IPLB-Sf9, highlighting the distinctions between the two. Importantly, the IOZCAS-Sf-1 cells exhibited a remarkable ability to generate polyhedra when infected with AcMNPV and SfMNPV, respectively. Consequently, this cellular lineage is considered a promising and valuable resource. It serves not only to investigate the molecular mechanisms of viral replication and its impact on host cells, but also to explore the transfection efficiency of SfMNPV DNA. This exploration further expands into its potential application in recombinant DNA experiments, laying a theoretical groundwork for the advancement of more effective biopesticides and sustainable agricultural practices.

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