LI Gang, ZHANG Peng-Fei, BANG Pei-Xia, WANG Gui-Qiu and LU Bao-Lan. APPLICATION OF RADIOIMMUNOPRECIPITATION TO THE TEST OF MONOCLONAL ANTIBODIES OF INFLUENZA VIRUS[J]. Virologica Sinica, 1986, 1(1).
Citation:
LI Gang, ZHANG Peng-Fei, BANG Pei-Xia, WANG Gui-Qiu, LU Bao-Lan.
APPLICATION OF RADIOIMMUNOPRECIPITATION TO THE TEST OF MONOCLONAL ANTIBODIES OF INFLUENZA VIRUS .VIROLOGICA SINICA, 1986, 1(1)
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摘要
本文报道了用放射免疫沉淀自显影技术,对流感病毒 A/洛阳/3/57(H_1N_1)单克隆抗体的鉴定工作。用流感病毒免疫 BALB/C 小鼠的脾细胞和小鼠骨髓瘤细胞(SPR/o)融合制备的单克隆抗体,此抗体经血凝抑制实验和神经氨酸酶抑制实验证明是对血凝素特异的。用蛋白 A—琼脂糖与单克隆抗体结合后,再与~(35)S 标记的病毒抗原结合,解析后,进行 SDS—聚丙烯酰胺凝胶电泳。放射自显影后,图谱呈现二条主带与 HA_1、HA_2的位置相符,即证明此单克隆抗体是对血凝素特异的。本文还就蛋白 A—琼脂糖对 IgG 的特异性结合及避免杂抗原带的出现等问题进行了讨论。
APPLICATION OF RADIOIMMUNOPRECIPITATION TO THE TEST OF MONOCLONAL ANTIBODIES OF INFLUENZA VIRUS
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Abstract
This article reports how we applied redioautography of immunopre- cipitation to the test of monoclonal antibodies of influenza virus A/Luo Yang/3/57(H_1N_1).The monoclonal antibodies were prepared from the fusion of the spleen cells of BALB/C mice,immunized with influenza vi- rus,and the myeloma ceils of SPR/O mice.These antibodies were proved specific to hemagglutinin through hemagglutination inhibition test and neuraminidase inhibit on test.The monoclonal antibodies combined pro- tein A-Sepharose then wit...
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References
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