IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

DING Da-Ming; WU Ya-Ming; FANG Ci-Qi; ZHOU Yong-Zhi; SUN Guo-Ping; ZHANG Yi-Min; JIAN Hao-Ran

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February 1986

DING Da-Ming, WU Ya-Ming, FANG Ci-Qi, ZHOU Yong-Zhi, SUN Guo-Ping, ZHANG Yi-Min and JIAN Hao-Ran. IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS[J]. Virologica Sinica, 1986, 1(1).

Citation: DING Da-Ming, WU Ya-Ming, FANG Ci-Qi, ZHOU Yong-Zhi, SUN Guo-Ping, ZHANG Yi-Min, JIAN Hao-Ran. IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS .VIROLOGICA SINICA, 1986, 1(1) : 31.

油桐尺蠖核型多角体病毒DNA片段的分子克隆及其鉴定

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

使用限制性核酸内切酶 BamHI 将油桐尺蠖核型多角体病毒(Buzura SuppressariaNuclear Polyhedrosis Virus。简称 BsNPV)DNA 切为9个片段。通过聚丙烯酰胺凝胶电泳洗脱,将其酶切片段与 BamHI 切割后的质粒 pBR_(322)重组,转化大肠杆菌,经抗菌素筛选、电泳分折和菌落杂交3种方法证明了插入子的存在,从而表明油桐尺蠖核型多角体病毒 DNA BamHI 片段已经克隆。

IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

IDENTIFICATION AND MOLECULAR CLONING OF DNA OF BUZURA SUPPRESSARIA NUCLEAR POLYHEDROSIS VIRUS

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: The DNA of Buzura Suppressaria Nuclear Polyhedrosis Virus(BsNPV) was digested at least9 fragments by restriction endonuclease BamHI.The fragments eluted by polyacrylamide gel electrophoresis were recombined with plasmid pBR_(322)incubated with BamHI and transfered into E.coli. Antibiotic screening,electrophoresis analysis and colony hybridization showed the existence of insert sequence in hybrid plasmid.The result suggested that the cloning of BamHI fragments of BsNPV DNA be successful.