STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS

QI Yi-Peng; G.Stainrs

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February 1986

QI Yi-Peng and G.Stainrs. STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS[J]. Virologica Sinica, 1986, 1(1).

Citation: QI Yi-Peng, G.Stainrs. STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS .VIROLOGICA SINICA, 1986, 1(1) : 36.

大蜡螟(Galleria mellonella)核型多角体病毒蛋白水解酶的研究

齐义鹏 ,
G.Stainrs

1.
武汉大学病毒学系
2.
美国俄亥俄州立大学昆虫学系武汉

摘要

用广泛的 pH 和不同的离子强度、温度研究了大蜡螟核型多角体病毒的多角体降解动力学,证明在病毒中存在一种蛋白水解酶,最适 pH 是8.8。以硫酸铵沉淀和 Sephacryl 柱层析分离纯化,酶活力提高5倍,冷冻干燥得白色絮状的纯化酶。层析图谱上得到3个峰。峰1是酶活力主峰,峰2仅以产生可溶性蛋白、峰3仅以降解多角体为主。由这三个组分的共同作用,多角体降解,病毒粒子释放,感染发生。电泳证明,降解的多角体产生两条带,P36可能是多角体蛋白的主要结构多肽,酶的反应速度是酶浓度的涵数,绘图得一级动力学曲线。

STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS

QI Yi-Peng ,
G.Stainrs

1.
Dept of Virology
2.
Wuhan UniversityG.Stainrs Dept of Entomology

Abstract

Dissolve kinetics for polyhedral matrix protein of Galleria mellonella nuclear polyhedrosis virus was investigated with wide range of pH,diff- erent ionic strength and temperature.A proteolytic enzyme was confir- med with the viral polyhedron.Its optimum pH for dissolved polyhedron is 8.8. The enzymes were purified by precipitaton with(NH_4)_2SO_4 and chrom- atograph with Sephacryl in result a five fold increase of relative enzyme activity.It was lyopholyzed to be a white cotton-form.Three different active ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

STUDY OF PROTEOLYTIC ENZYME ASSOCIATED WITH THE POLYHEDRAL PROTEIN OF Galleria mellonella NUCLEAR POLYHEDROSIS VIRUS

QI Yi-Peng ,
G.Stainrs

1. Dept of Virology
2. Wuhan UniversityG.Stainrs Dept of Entomology

Abstract: Dissolve kinetics for polyhedral matrix protein of Galleria mellonella nuclear polyhedrosis virus was investigated with wide range of pH,diff- erent ionic strength and temperature.A proteolytic enzyme was confir- med with the viral polyhedron.Its optimum pH for dissolved polyhedron is 8.8. The enzymes were purified by precipitaton with(NH_4)_2SO_4 and chrom- atograph with Sephacryl in result a five fold increase of relative enzyme activity.It was lyopholyzed to be a white cotton-form.Three different active ...