ZHANG Tian-Ming, XIANG Jin-Min and FENG Jue-Sun. ON THE SDS-PAGE ANALYSIS OF 4 TYPES OF CELL CULTURE INFECTED WITH 4 STRAINS OF HERPES SIMPLEX VIRUS[J]. Virologica Sinica, 1986, 1(2).
Citation:
ZHANG Tian-Ming, XIANG Jin-Min, FENG Jue-Sun.
ON THE SDS-PAGE ANALYSIS OF 4 TYPES OF CELL CULTURE INFECTED WITH 4 STRAINS OF HERPES SIMPLEX VIRUS .VIROLOGICA SINICA, 1986, 1(2)
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四株单纯疱疹病毒(HSV)感染四种细胞的SDS-PAGE分析
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1.
湖北医学院病毒研究所
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2.
湖北医学院病毒研究所 武昌
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摘要
我们用 SDS—PAGE 测定四株 HSV 感染 HEp—2细胞、HeLa 细胞、人胚肺细胞、鸡胚细胞多肽与未感染细胞多肽的电泳图结果表明:四小时感染细胞多肽与未感染细胞多肽相比未见有区别,二十四小时的感染细胞多肽与未感染细胞多肽的电泳带显示有所区别。其中感染 HSV—1和 HSV—2型的鸡胚细胞多肽电泳带亦显示有所区别。说明鸡胚细胞对 HSV—1型和 HSV—2型的敏感性差异,亦可以从电泳带反映出来,说明电泳带可作为 HSV 分型的又一方法。经蔗糖梯度超离心纯化的未标记同位素的地方株 HSV—1CC21株和 HSV—2W 株病毒颗粒用 SDS 裂解,并进行 SDS—PAGE 表明这两株病毒的结构多肽分别为12和15种,显示型的区别。本文还就关于在制备疫苗时以选择何种细胞为宜的问题进行了讨论。
ON THE SDS-PAGE ANALYSIS OF 4 TYPES OF CELL CULTURE INFECTED WITH 4 STRAINS OF HERPES SIMPLEX VIRUS
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1.
Virus Research Institute
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Hubei Medical College
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Abstract
Four types of cell culture,namely,HEP-2,HeLa,human embryo lung fibroblast and chicken embryo fibroblast were infected with 4 st- rains of herpes simplex virus,i.e.CC21,F,W,and G,the formal 2 of type 1 and W and G of type 2.The SDS-PAGE maps showed dif- ference between the maps form at 4h after infection and that at twenty four hours after virus infection,the electrophoresis maps showed a dif- ference between infected and uninfected cells.In the chicken embryo fi- broblast culture,there were difference betwe...
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