A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

LUO Jing; WANG Han-Zhong; YANG Xue-Lou; WU Xi-Ying; HUANG Wen-Lin

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April 1986

LUO Jing, WANG Han-Zhong, YANG Xue-Lou, WU Xi-Ying and HUANG Wen-Lin. A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES[J]. Virologica Sinica, 1986, 1(2).

Citation: LUO Jing, WANG Han-Zhong, YANG Xue-Lou, WU Xi-Ying, HUANG Wen-Lin. A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES .VIROLOGICA SINICA, 1986, 1(2) : 24.

薄荷伪造桥虫多角体病毒及其核酸的限制性内切酶酶解分析

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

本文描述了从薄荷伪造桥虫(Argrogramma agnata stgr.)幼虫分离到的一种核型多角体病毒的形态以及采用简便的方法提取的核酸,经限制性内切酶 EcoRI,BamHI,HindⅢ,BglⅠ,BglⅡ和BglⅠ+BglⅡ,BamHl+EcoRl 酶解,获得该病毒核酸的酶解带谱。以入 DNA 的 EcoRl 酶解片段在凝胶中的迁移率与相应 DNA 片段分子量的对数值作标准曲线。从曲线上求得薄荷伪造桥虫多角体病毒核酸酶解各片段的分子量.此病毒核酸的平均分子量为108.51×10~6道尔顿。

A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

1.
Wuhan Insititute of Virology
2.
Academia Sinica

Abstract

In this paper the morphology of NPV isolated from A.a.Stgr.is des- cribed and the map of A.a.NPV-DNA extracted in a simple method is obtained through restriction endonuclease digest such as EcoRI,BglⅠ, BglⅡ,BamHI,HindⅢ,and double digest BglⅠ+BglⅡ,BamHI+EcoRI.By using relationship between the different size of λ-DNA cleaved by EcoRI and it s mobility in 1% agarose electrophosis,standard curve of molec- ular weight is made.The molecular weight of each A.a.NPV-DNA fragments generated by restriction endonucleas...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

A STUDY ON THE NPV OF ARGROGRAMMA AGNATA STGR AND ANALYSIS OF ITS NUCLEAR ACID MAP WITH RESTRICTION ENDONUCLEASES

1. Wuhan Insititute of Virology
2. Academia Sinica

Abstract: In this paper the morphology of NPV isolated from A.a.Stgr.is des- cribed and the map of A.a.NPV-DNA extracted in a simple method is obtained through restriction endonuclease digest such as EcoRI,BglⅠ, BglⅡ,BamHI,HindⅢ,and double digest BglⅠ+BglⅡ,BamHI+EcoRI.By using relationship between the different size of λ-DNA cleaved by EcoRI and it s mobility in 1% agarose electrophosis,standard curve of molec- ular weight is made.The molecular weight of each A.a.NPV-DNA fragments generated by restriction endonucleas...