WU Jian-Hua, LIU Hui-Hua, CHEN Zuo-Yi, GONG Zu-Xun, LIN Rui-Fen and JIN Deng-Di. STUDIES ON PURIFICATION, MORPHOLOGY AND DOUBLE STRANDED RNA OF RICE RAGGED STUNT VIRUS[J]. Virologica Sinica, 1986, 1(3).
Citation:
WU Jian-Hua, LIU Hui-Hua, CHEN Zuo-Yi, GONG Zu-Xun, LIN Rui-Fen, JIN Deng-Di.
STUDIES ON PURIFICATION, MORPHOLOGY AND DOUBLE STRANDED RNA OF RICE RAGGED STUNT VIRUS .VIROLOGICA SINICA, 1986, 1(3)
: 69.
我国水稻齿叶矮缩病毒的分离提纯及病毒形态和核酸的研究
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1.
中国科学院上海生物化学研究所
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2.
浙江农业科学院病毒研究室
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3.
浙江农业科学院病毒研究室 上海
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摘要
应用比较简单的一次四氯化碳澄清,二次聚乙二酵浓缩沉淀,差速离心和20%—50%的蔗糖密度梯度离心,可以获得分离提纯效果较好的水稻齿叶病病毒(RRSV)制剂,在260nm处有最大吸收值,在A_(260)/A_(280)的比值为1.6。用醋酸铀负染方法,可以观察到RRSV为直径54nm—65nm的球状颗粒,具有底部较宽的突起,突起的高度为8—11nm,宽度约为22nm。病毒的核酸为双链RNA,共有十组分散的基因组,这一结果和四方报道的一致,在这一提纯病毒的制剂中,尚发现有其他三种大小不同的球状和线状颗粒,对它们的可能来源进行了讨论。
STUDIES ON PURIFICATION, MORPHOLOGY AND DOUBLE STRANDED RNA OF RICE RAGGED STUNT VIRUS
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Abstract
The partial Purified preparations of Rice Ragged Stunt Virus (RRSV) from fresh diseased rice leaves could be obtained in the high yield by the combination of the techniques of clarification with CCI_4, precipitation with Polyethylene Glycol,differential centrifugations and linear sucrose gradient centrifugations. The preparations showed the maxium of the ultra-violet absorption spectra was at 260 nm and the value of the A_(260)/A_(280) ratio was 1.6. By Electron microscopy the RRSV particles showed spiked i...
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References
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Proportional views
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