ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

LI Cheng-Ming; CHEN Li-Li; YIN Da-Chang; CHEN Dan-Lin; LIU Li-Hua; WANG Hong-Xi; HU Ping

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August 1986

LI Cheng-Ming, CHEN Li-Li, YIN Da-Chang, CHEN Dan-Lin, LIU Li-Hua, WANG Hong-Xi and HU Ping. ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO[J]. Virologica Sinica, 1986, 1(4).

Citation: LI Cheng-Ming, CHEN Li-Li, YIN Da-Chang, CHEN Dan-Lin, LIU Li-Hua, WANG Hong-Xi, HU Ping. ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO .VIROLOGICA SINICA, 1986, 1(4) : 30.

甲型肝炎病毒分离及培养特性的研究

1.
四川省卫生防疫站病毒性疾病控制科
2.
四川省卫生防疫站病毒性疾病控制科成都

摘要

应用人胚肺二倍体细胞(2BS)直接从甲肝病人急性期粪便中分离培养出一株甲型肝炎病毒(S_(84=1))。该毒株无致细胞病变作用,具有产量和毒力较高(10~(-6.5～(-7)))繁殖周期短(7天左右)的优点,可作为生产甲型肝炎诊断试剂和研制甲型肝炎疫苗的侯选毒株。

ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

1.
Department of Viral Disease of Control
2.
Health and Anti-epldemic Station of Sichuan Province

Abstract

A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ISOLATION AND PROPAGATION OF HEPATITIS A VIRUS IN VITRO

1. Department of Viral Disease of Control
2. Health and Anti-epldemic Station of Sichuan Province

Abstract: A Strain of hepatitis A virus(S_(84-1))was directly isolated from a fecal specimen of acute patient with hepatitis A and serially passaged in human diploid fibroblast(2BS). Some conditions of cultivation of this virus were also studied. Following serial propagation, the incubation time of S_(84-1) virus was reduced from one and half months(passage 2) to 3 days(passage 12). S_(84-1) virus, without cytopathie effect, can t be detected in supernatant fluid by ELISA and was harvested by freezing-throwing the ce...