GAO Qian, JIANG Chao-Zhun, MA Wen-Yu, ZHONG Fang-Yang, CA Xiao-Ning and HONG Mei-Xian. THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES[J]. Virologica Sinica, 1986, 1(4).
Citation:
GAO Qian, JIANG Chao-Zhun, MA Wen-Yu, ZHONG Fang-Yang, CA Xiao-Ning, HONG Mei-Xian.
THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES .VIROLOGICA SINICA, 1986, 1(4)
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抗HSV-1淋巴细胞杂交瘤的建立及应用单克隆抗体对HSV分型
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第四军医大学微生物教研室
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广州第一军医大学南方医院免疫室
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西安医学院附属二院妇产科
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第四军医大学微生物教研室 西安
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摘要
应用HSV-1 SM44株感染的BHK-21细胞及提取的感染细胞膜蛋白抗原免疫Balb/c小鼠。以免疫小鼠脾细胞与小鼠骨髓瘤细胞系Sp_2/0融合,经ELISA筛选仅与HSV-1反应的阳性克隆及克隆化,建立了一株产生抗HSV-1型特异性McAb的杂交瘤细胞系(Mad-2)。经ELISA,IF及抗原吸收试验证明,该细胞系的培养上清及制备的小鼠腹水均与HSV-1呈特异性反应,但不与HSV-2反应。ELISA测定McAb Mad-2的腹水效价为10~(-7)Ig亚类鉴定为IgG-1。应用Mad-2和抗HSV型共同性McAb 1A12及抗HSV-2型特异性McAb CH-A9,对43株临床HSV分离株做了ELISA及IF分型。结果表明,28株口唇、眼部感染分离株和1株宫颈炎分离株均与1A12和Mad-2反应,不与CH-A9反应,为HSV-1。余14株富颈炎分离株均与1A12和CH-A9反应,而不与Mad-2反应,为HSV-2。ELISA和IF分型的结果完全一致。本研究提出作者应用的一套抗HSVMcAb有可能作为HSV型别鉴定的标准试剂。
THE PRODUCTION OF AN ANTI-NSV-1 LYMPHOCYTE HYBRIDOMA CELL LINE AND TYPING OF HSV ISOLATES WITH MONOCLONAL AHTIBODIES
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Abstract
A hybridoma cell line(Mad-2) was produced by fusing mouse myloma cell line Sp2/O with the spleen cells immunized by HSV-1 SM44 strain infected BHK-21 cells and membrane protein antigen extracted from the HSV-1 infected ceils, the positive clone Mad-2 secreating monoclonal antibody(McAb) against HSV-1 was selected by ELISA. the supernatant and ascites producet by Mad-2 all reacted with HSV-1, hut not with HSV-2. the titer of McAb in ascites mesured by ELISA was 10~(-7) and the subclass of the Mad-2 was IgG1....
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