XIA Dong-Xiang, WANG Mei-Xian, NI Da-Shi, JIANG Shao-Chun and MA Wen-Yu. RESEARCHES ON THE HEMAGGLNTININ(HAN) OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRSV) Ⅱ. An Investigation of Factors Involving HA and HI test[J]. Virologica Sinica, 1986, 1(4).
Citation:
XIA Dong-Xiang, WANG Mei-Xian, NI Da-Shi, JIANG Shao-Chun, MA Wen-Yu.
RESEARCHES ON THE HEMAGGLNTININ(HAN) OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRSV) Ⅱ. An Investigation of Factors Involving HA and HI test .VIROLOGICA SINICA, 1986, 1(4)
: 49.
肾综合征出血热病毒血凝素的研究 Ⅱ.血凝和血凝抑制试验影响因素的探讨
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1.
第四军医大学微生物学教研室
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安徽省医学科学研究所病毒室
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3.
第四军医大学微生物学教研室 西安
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摘要
本文探讨了肾综合征出血热病毒(HFRSV)血凝和血凝抑制(简称血抑)试验的主要影响因素,观察到HFRSV血凝素的制备要求较高,4℃保存的效果较好,血凝素对热似有一定的稳定性,血凝作用的条件较为严格,最佳pH在5.76.1,鹅血球较为敏感,氯仿法去除血清中非特异性抑制物有效而简便。通过试验条件的模索和方法的改进,使之适用于基层。
RESEARCHES ON THE HEMAGGLNTININ(HAN) OF HEMORRHAGIC FEVER WITH RENAL SYNDROME(HFRSV) Ⅱ. An Investigation of Factors Involving HA and HI test
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Abstract
In this paper, the factors involving HA and HI test were reported It was observed that the preparation of HFRSV-HAN and the conditions for HA test were strict. The HAN was preserved well at 4℃ and seemed tolerabe to heat to some degree. The optimal pH ranged from 5.7 to 6.1, and the goose erythrocyte was most sensitive to the HAN among 4 sorts of red blood cells. The chloroform method for the removal of non-specific inhibitors from serum was proved both effective and convenient. It is concluded that with th...
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References
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Proportional views
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