SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES

ZHANG Qi-Ya; LIU Yong; YANG Ping; LIN Mu-Lan; WENG Zhao-Kui; MI Ke-Yong; ZHUO Zhong-Qi; DING Da-Ming; YANG Xi-Cai; CHEN Wei; TIAN Bo

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August 1986

ZHANG Qi-Ya, LIU Yong, YANG Ping, LIN Mu-Lan, WENG Zhao-Kui, MI Ke-Yong, ZHUO Zhong-Qi, DING Da-Ming, YANG Xi-Cai, CHEN Wei and TIAN Bo. SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES[J]. Virologica Sinica, 1986, 1(4).

Citation: ZHANG Qi-Ya, LIU Yong, YANG Ping, LIN Mu-Lan, WENG Zhao-Kui, MI Ke-Yong, ZHUO Zhong-Qi, DING Da-Ming, YANG Xi-Cai, CHEN Wei, TIAN Bo. SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES .VIROLOGICA SINICA, 1986, 1(4) : 93.

柑桔裂皮类病毒和菊花矮化类病毒互补DNA探针的合成及检测

1.
中国科学院武汉病毒研究所
2.
中国科学院微生物研究所
3.
中国科学院微生物研究所武汉

摘要

分别用人工合成的柑桔裂皮类病毒和菊花矮化类病毒的互补DNA探针,经固相分子杂交(dot blot)检测了柑桔、菊花等样品。结果表明:与生物学检测和聚丙烯酰胺凝胶电泳(PAGE)分析相比,这是早期诊断类病毒病和检测类病毒的一种较为灵敏、快速的方法。

SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES

1.
Wuhan Institute of Virology Academia Sinica
2.
WuhanInstitute of Microbiology Academia Sinica
3.
Beijing

Abstract

The ~(32)P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology assays and polyacrylamide gel electrophoresis,

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

SYNTHESIS OF CITRUS EXOCORTIS VIROID AND CHRYSANTHEMUM STUNT VIROID DNA ~(32)P-cDNA PROBES AND DETECTION BY THE PROBES

1. Wuhan Institute of Virology Academia Sinica
2. WuhanInstitute of Microbiology Academia Sinica
3. Beijing

Abstract: The ~(32)P-cDNA Probes of Citrus Exocortis Viroid (CEV) and Chrysanthemum Stunt Viroid (CSV)RNA has been synthesied respectively and usedfor detecting some samples that may contain relative viroids. This is a sensitive and rapid method for diagnosing early viroid disease as compared with biology assays and polyacrylamide gel electrophoresis,