ESTABLISHMENT OF EXPERIMENTAL PERSISTENT INFECTION OF MOUSE CYTOMEGALOVIRUS MODEL

HU Chun-Da; WEN Yu-Mei

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February 1987

HU Chun-Da and WEN Yu-Mei. ESTABLISHMENT OF EXPERIMENTAL PERSISTENT INFECTION OF MOUSE CYTOMEGALOVIRUS MODEL[J]. Virologica Sinica, 1987, 2(1).

Citation: HU Chun-Da, WEN Yu-Mei. ESTABLISHMENT OF EXPERIMENTAL PERSISTENT INFECTION OF MOUSE CYTOMEGALOVIRUS MODEL .VIROLOGICA SINICA, 1987, 2(1) : 28.

小鼠巨细胞病毒实验性持续感染模型的建立

胡纯达 ,
闻玉梅

1.
上海医科大学微生物教研室
2.
上海医科大学微生物教研室上海

摘要

应用小鼠巨细胞病毒(MCMV)Smith株,经腹腔感染我国繁殖的C57BL/6近交系小鼠,在高剂量感染组(10~(5.33)TCID_(50))、中剂量感染组(2×10~(4.33)TCID_(50))、低剂量感染组(2×10~(3.33)TCID_(50))均可引起感染。自感染后第5—14天,所有感染组鼠脾脏均可100％分离到病毒。自第14天开始,高、中剂量感染组唾液腺中已可100％分离到病毒;低剂量感染组唾液腺中到21天亦可全部分离到病毒;中剂量感染组小鼠追踪至第102天,唾液腺中仍100％可分离到病毒。MCMV感染后第21天的小鼠唾液腺切片中,还可见到腺体细胞核增大,核质疏松,有些细胞可见核内包涵体。本研究中全部对照小鼠均未分离到MCMV,说明本研究所用的C57BL/6小鼠可进行实验MCMV感染的研究。

ESTABLISHMENT OF EXPERIMENTAL PERSISTENT INFECTION OF MOUSE CYTOMEGALOVIRUS MODEL

HU Chun-Da ,
WEN Yu-Mei

1.
Department of Microbiology
2.
Skanghoi Medical Univ. Shanghai

Abstract

Smith strain of murine cytomegalovirus (MCMV) was used for intraperitoneal infection of C57BL/6 inbred mice. in all three groups of high(10~(5.33)TCID_(50)), medium (2×10~(4.33)TCID_(50))and low (2×10~(3.33)TCID_(50)) dosage infected mice, MCMV infection was successfully established During 5-14 days after infection, in all dosage groups MCMV could be isolated from the spleens Starting from the 14th day after infection, salivary glands from all mice of the high and medium dosage groups were MCMV positive, wh...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

ESTABLISHMENT OF EXPERIMENTAL PERSISTENT INFECTION OF MOUSE CYTOMEGALOVIRUS MODEL

HU Chun-Da ,
WEN Yu-Mei

1. Department of Microbiology
2. Skanghoi Medical Univ. Shanghai

Abstract: Smith strain of murine cytomegalovirus (MCMV) was used for intraperitoneal infection of C57BL/6 inbred mice. in all three groups of high(10~(5.33)TCID_(50)), medium (2×10~(4.33)TCID_(50))and low (2×10~(3.33)TCID_(50)) dosage infected mice, MCMV infection was successfully established During 5-14 days after infection, in all dosage groups MCMV could be isolated from the spleens Starting from the 14th day after infection, salivary glands from all mice of the high and medium dosage groups were MCMV positive, wh...