PRELIMINARY STUDY OF MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUSES AMONG INFANTS WITH ACUTE GASTROENTERITIS IN SHANGHAI, 1982—1984

ZHOU Yong-Jie; JIANG Hui-Hui; CAO Shi-Fang; CUI Gui-Fang; HONG Jing; SHI Yun-Cheng

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February 1987

ZHOU Yong-Jie, JIANG Hui-Hui, CAO Shi-Fang, CUI Gui-Fang, HONG Jing and SHI Yun-Cheng. PRELIMINARY STUDY OF MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUSES AMONG INFANTS WITH ACUTE GASTROENTERITIS IN SHANGHAI, 1982—1984[J]. Virologica Sinica, 1987, 2(1).

Citation: ZHOU Yong-Jie, JIANG Hui-Hui, CAO Shi-Fang, CUI Gui-Fang, HONG Jing, SHI Yun-Cheng. PRELIMINARY STUDY OF MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUSES AMONG INFANTS WITH ACUTE GASTROENTERITIS IN SHANGHAI, 1982—1984 .VIROLOGICA SINICA, 1987, 2(1) : 51.

1982—1984年上海市秋冬季婴幼儿急性胃肠炎病例中人轮状病毒分子流行病学的研究

1.
上海医科大学公共卫生学院
2.
中科院上海生化研究所
3.
上海市工农医院
4.
上海市儿童医院上海
5.
上海

摘要

1982—1984年上海市二个医院收集的小儿腹泻标本150份,用RNAPAGE银染法分析轮状病毒RNA迁移图谱;在阳性标本74例中72例为2L电泳型,2例不能分为2L或2S电泳型。RNA电泳带迁移差别较明显的为2、3和7、8、9RNA片段。根据7、8、9三条带迁移率之不同,将RNA迁移图谱分成3种电泳亚型。统计学检验说明82年以第2电泳亚型为主,83年以第1电泳亚型为主,84年均为第1电泳亚型(P<0.05)。提示82—84年RNA迁移图谱有明显的变迁。二个医院相距甚远,但每年的RNAPACE图谱分布趋势基本一致。

PRELIMINARY STUDY OF MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUSES AMONG INFANTS WITH ACUTE GASTROENTERITIS IN SHANGHAI, 1982—1984

1.
Shanghai Medical University
2.
ShanghaiShanghai Institute of Biochemistry
3.
Academia Sinica
4.
ShanghaiShanghai Hospital for Wokers and Peasants
5.
ShanghaiShanghai Children s Hospital

Abstract

150 stool samples were collected from acute infantile gastroenteritis patients in two hospitals in Shanghai from 1982 to 1984. The migration patterns of rotavirus RNA genome segments in polyacrylamide gel electrophoresis(PAGE)were compared by silver-staining. RNA PAGE showed that 74 samples were positive, among which 72 samples were of long electropherotype (2L), and 2 samples could not be categorized into 2S or 2L. Significant differences could be detected in some of the 2. 3. 7. 8 and 9 segments. Accordin...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

PRELIMINARY STUDY OF MOLECULAR EPIDEMIOLOGY OF HUMAN ROTAVIRUSES AMONG INFANTS WITH ACUTE GASTROENTERITIS IN SHANGHAI, 1982—1984

1. Shanghai Medical University
2. ShanghaiShanghai Institute of Biochemistry
3. Academia Sinica
4. ShanghaiShanghai Hospital for Wokers and Peasants
5. ShanghaiShanghai Children s Hospital

Abstract: 150 stool samples were collected from acute infantile gastroenteritis patients in two hospitals in Shanghai from 1982 to 1984. The migration patterns of rotavirus RNA genome segments in polyacrylamide gel electrophoresis(PAGE)were compared by silver-staining. RNA PAGE showed that 74 samples were positive, among which 72 samples were of long electropherotype (2L), and 2 samples could not be categorized into 2S or 2L. Significant differences could be detected in some of the 2. 3. 7. 8 and 9 segments. Accordin...