KE Li-Hua and WU Yuan-Ming. LIBRARY AND PHYSICAL MAP OF HELIOTHIS ARMIGERA NUCLEAR POLYHEDROSIS VIRUS DNA[J]. Virologica Sinica, 1987, 2(1).
Citation: KE Li-Hua, WU Yuan-Ming. LIBRARY AND PHYSICAL MAP OF HELIOTHIS ARMIGERA NUCLEAR POLYHEDROSIS VIRUS DNA .VIROLOGICA SINICA, 1987, 2(1) : 57.

棉铃虫核多角体病毒基因库及其物理图谱

  • 本文报道棉铃虫核多角体病毒DNA经限制性内切酶BamHI酶解,琼脂糖凝胶电泳分离,得到大小不同的11种片段。所得片段与BamHI酶解的pBR_(322)质粒DNA进行体外重组并转化大肠杆菌LE392菌株。根据菌落杂交和插入片段的分子量等鉴定,证明获得11个插入了病毒DNA片段的重组质粒,但其中两个大片段克隆的分子量比原片段小。通过限制酶EcoRI和BamHI酶解片段的交叉吸印杂交及用~(32)p标记的克隆片段与病毒DNA酶解片段杂交等方法,构建了病毒基因组BamHI的物理图谱。杂交结果表明,病毒基因组主要由独特的序列组成。

LIBRARY AND PHYSICAL MAP OF HELIOTHIS ARMIGERA NUCLEAR POLYHEDROSIS VIRUS DNA

  • Heliothis armigera polyhedrosis virus DNA was cleaved into 11 fragments with BamH1 which were isolated by using agarose gel electrophoresis. The resulting fragments and BamH1 digestion products of PBR_(322) DNA were used to recombine in vitro and to transform E. coli strain LE_(392). Based on the identification of colony hybridization and molecular weights of inserted fragments, we have obtained 11 recombinant plasmids inserting viral DNA, but two of molecular weights for the largest fragments are less than...

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    LIBRARY AND PHYSICAL MAP OF HELIOTHIS ARMIGERA NUCLEAR POLYHEDROSIS VIRUS DNA

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    Abstract: Heliothis armigera polyhedrosis virus DNA was cleaved into 11 fragments with BamH1 which were isolated by using agarose gel electrophoresis. The resulting fragments and BamH1 digestion products of PBR_(322) DNA were used to recombine in vitro and to transform E. coli strain LE_(392). Based on the identification of colony hybridization and molecular weights of inserted fragments, we have obtained 11 recombinant plasmids inserting viral DNA, but two of molecular weights for the largest fragments are less than...

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