BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO

ZHANG Zhong-Xin; BANG Hui-Yin; JIN Feng; ZHANG Yang-Lian; WANG Lu-Ming; ZENG Yun-Tian

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April 1987

ZHANG Zhong-Xin, BANG Hui-Yin, JIN Feng, ZHANG Yang-Lian, WANG Lu-Ming and ZENG Yun-Tian. BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO[J]. Virologica Sinica, 1987, 2(2).

Citation: ZHANG Zhong-Xin, BANG Hui-Yin, JIN Feng, ZHANG Yang-Lian, WANG Lu-Ming, ZENG Yun-Tian. BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO .VIROLOGICA SINICA, 1987, 2(2) : 60.

体外组织培养增殖的油桐尺蠖核型多角体病毒毒力的生物测定

1.
中国科学院武汉病毒研究所

摘要

活体增殖和体外组织培养增殖的BsNPV悬液稀释成不同浓度,分别喷于人工饲料上,使其自然渗入饲料内,接3龄幼虫取食感染,感染剂量与幼虫死亡率的回归直线方程分别为;y=3.1815+0.679x和y=3.2000+0.665x,由回归直线方程推算的LD_(50)值及95％置信界限为4.0753×10~2[9.6075×10~2][1.7301×10~2]PIB/克饲料及5.0898×10~2[1.2411×10~3][2.0874×10~2]PIB/克饲料。感染剂量为4×10~5PIB/克饲料的LT_(50)值分别为4.7168天和4.8083天。比较两种来源的BsNPV,其多角体的感染力无明显差异。

BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO

1.
Wuhan Institute of Virology

Abstract

Bioassay of a Nuclear Polyhedrosis Virus propagated either in vivo or vitro was conducted on third-instar Buzura suppressaria larvae which were reared on an artificial diet contaminated with virus, The regression equation between dosage and mortality were:Y=3.1815+0.697XY=3.2000+0.665XThe LD_(50) value and 95％ fiducial limits were 4.0753×10~2 (9.0675×10~2)(1.7361×10~2)PIB/g of diet and 5.0898×10~2(1.2411×10~3)(2.0874×10~2) PIB/g of diet,respectively.The LT_(50) value for 4×10~5PIB/g of diet were 4.7168 days...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

BIOASSAY OF A NUCLEAR POLYHEDROSIS VIRUS OF THE TEA LOOPER, BUZURA SUPPRESSARIA, PROPAGATED IN CELLS GROWN IN VITRO

1. Wuhan Institute of Virology

Abstract: Bioassay of a Nuclear Polyhedrosis Virus propagated either in vivo or vitro was conducted on third-instar Buzura suppressaria larvae which were reared on an artificial diet contaminated with virus, The regression equation between dosage and mortality were:Y=3.1815+0.697XY=3.2000+0.665XThe LD_(50) value and 95％ fiducial limits were 4.0753×10~2 (9.0675×10~2)(1.7361×10~2)PIB/g of diet and 5.0898×10~2(1.2411×10~3)(2.0874×10~2) PIB/g of diet,respectively.The LT_(50) value for 4×10~5PIB/g of diet were 4.7168 days...