DETECTING EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN AND ANTIBODY BY IMMUNE ADHERENCE HEMAGGLUTINATION(IAHA) TEST

DONG Guan-Mu; LUO Hui-Rong; LIU Wen-Xue; CHEN Ming; SHU Yong-Xin

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August 1987

DONG Guan-Mu, LUO Hui-Rong, LIU Wen-Xue, CHEN Ming and SHU Yong-Xin. DETECTING EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN AND ANTIBODY BY IMMUNE ADHERENCE HEMAGGLUTINATION(IAHA) TEST[J]. Virologica Sinica, 1987, 2(4).

Citation: DONG Guan-Mu, LUO Hui-Rong, LIU Wen-Xue, CHEN Ming, SHU Yong-Xin. DETECTING EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN AND ANTIBODY BY IMMUNE ADHERENCE HEMAGGLUTINATION(IAHA) TEST .VIROLOGICA SINICA, 1987, 2(4) : 14.

应用免疫粘附血凝技术检测流行性出血热病毒抗原和抗体的试验研究

1.
卫生部药品生物制品检定所
2.
卫生部药品生物制品检定所北京

摘要

本文对免疫粘附血凝(IAHA)技术进行了改进并用于检测流行性出血热病毒搞原和血清效价。比较了不同毒株在Vero-E_6细胞内抗原滴度,表明A_9株的滴度最高达1:64,以IAHA法对出血热病人9份血清,3份恢复期血清和家兔免疫血清6份进行了效价测定。并同时用免疫荧光(IFA)法进行比较,结果抗体阳性率二种方法一致,但前者的抗体滴度和增长倍数较后者高2-64倍,以上结果表明IAHA法可代替IFA法用于出血热病人或感染动物的抗体检测。

DETECTING EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN AND ANTIBODY BY IMMUNE ADHERENCE HEMAGGLUTINATION(IAHA) TEST

1.
National Institute for the Control of Pharmaceutical and BiologicaI Products
2.
Beijing

Abstract

We have modified and improved the method of immune adherence hemagglutination (IAHA) test and used it to detect virus antigen and antibody of epidemic hemorrhagic fever (EHF). We compared the IAHA titers of different strains infected in Vero-E_6 cells The IAHA titer of A_9 strain showed to be the highest (1: 64) among the three strains. 9 EHF patients paired sera, 3 con valecant sera and 6 immunized rabbits sera were detected with IAHA and compared with IFA. The positive rates detected by the two tests we...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

DETECTING EPIDEMIC HEMORRHAGIC FEVER VIRUS ANTIGEN AND ANTIBODY BY IMMUNE ADHERENCE HEMAGGLUTINATION(IAHA) TEST

1. National Institute for the Control of Pharmaceutical and BiologicaI Products
2. Beijing

Abstract: We have modified and improved the method of immune adherence hemagglutination (IAHA) test and used it to detect virus antigen and antibody of epidemic hemorrhagic fever (EHF). We compared the IAHA titers of different strains infected in Vero-E_6 cells The IAHA titer of A_9 strain showed to be the highest (1: 64) among the three strains. 9 EHF patients paired sera, 3 con valecant sera and 6 immunized rabbits sera were detected with IAHA and compared with IFA. The positive rates detected by the two tests we...