XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei and WANG Hong-Xia. PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM[J]. Virologica Sinica, 1987, 2(4).
Citation:
XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei, WANG Hong-Xia.
PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM .VIROLOGICA SINICA, 1987, 2(4)
: 43.
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摘要
用自制的MC-1型微载体进行悬浮培养,当其浓度为5mg/ml时,可较好地用以培养和增殖鼠L929细胞。当接种细胞量为30×10~4/ml左右时,一般在3天即可增殖至1×10~6细胞/ml。用25IU/ml鼠IFN起动12—24小时,用NDV作诱生剂,并采用环己亚胺(20μg/ml)和放线菌素D(2μg/ml)进行超诱导,IFN产量可高达1×10~6IU/ml左右,比活接近1.3×10~5IU/ml蛋白。尽量去除培养基,加胰酶—柠檬酸盐消化和高速搅拌,使细胞从载体上分离,再加新鲜培养基和微载体的方法进行扩大生产看来是可行的。这些实验表明采用微载体悬浮培养细胞的技术将更适于IFN的工业化生产。
PILOT PRODUCTION OF HIGH-TITRE MOUSE INTERFERON WITH MICROCARRIER SUSPENSION CULTURE SYSTEM
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Abstract
With MC-1 type microcarrier at concentration of 5mg/ml, the mouse L929 cell line could attach and proliferate on them very well if the stirring speed was lower than 50r/m.When the cell density was inoculated about 30×10~4/ml,it could proliferate to 1×10~6/ml after 3 days generally. At this time,priming with 25 IU/ml mouse IFN about 12-24 h and then induced with NDV and superinduced with cycloheximide(20μg/ml) and actinomycin D(2μg/ml),the titer of IFN in the media would be 10~5IU/ml approximately, or 10~5IU...
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References
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Proportional views
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