THE MUTAGENESIS OF ATTENUATED STRAIN OF TMV DN60-3 AND THE PROTECTIVE EFFECT ON TOMATO MOSAIC DISEASE

ZHENG Gui-Bin

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August 1987

ZHENG Gui-Bin. THE MUTAGENESIS OF ATTENUATED STRAIN OF TMV DN60-3 AND THE PROTECTIVE EFFECT ON TOMATO MOSAIC DISEASE[J]. Virologica Sinica, 1987, 2(4).

Citation: ZHENG Gui-Bin. THE MUTAGENESIS OF ATTENUATED STRAIN OF TMV DN60-3 AND THE PROTECTIVE EFFECT ON TOMATO MOSAIC DISEASE .VIROLOGICA SINICA, 1987, 2(4) : 62.

TMV弱毒DN60—3的诱变及其对番茄花叶病的保护效果试验

郑贵彬

1.
陕西省西安市农业科学研究所西安

摘要

为获得TMV弱毒,在选择轻症纯毒系的基础上,以亚硝酸诱变处理30、40、50、60分钟,得到了30个无症突变株,经室内交叉保护试验,其中DN60-3对TMV的重花叶株系、条斑株系和山东济南的坏疽型都有显著的干扰作用,而对生产影响不大的黄色花叶株系和黄色环斑株系的干扰作用差。田间试验示范结果,对保护地栽培的番茄花叶病(包括卷叶病)的防病增产效果明显。同时发现,栽培技术差,植株长势弱,则保护放果减低。由于弱毒的保护作用属于持异性免疫,因此,在弱毒应用上,对TMV和CMV混发地区的露地番茄无保护作用。随着抗TMV番茄品种的全面推广,弱毒的利用将会失去重要性,目前一些中小城市番茄品种正处于更新时期,弱毒仍可发挥一定作用。

THE MUTAGENESIS OF ATTENUATED STRAIN OF TMV DN60-3 AND THE PROTECTIVE EFFECT ON TOMATO MOSAIC DISEASE

ZHENG Gui-Bin

1.
Xi'an Institute of Agriculture

Abstract

For the purpose of obtaining attenuated strain of TMV, pure virus isolated from tomato plants with mild symptom were treated with nitrous acid for 30, 40, 50, 60 minutes, as a result, 30 symptomless mutants had been obtained. In cross protection tests on tomato, the attenuated virus DN60-3 interfered more strongly with the severe mosaic strain, the streak strain and the necrosis strain occurred in Jinan (Shandong), whereas interfered weakly with the yellow mosaic strain and the yellow ringspot strain, both ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

THE MUTAGENESIS OF ATTENUATED STRAIN OF TMV DN60-3 AND THE PROTECTIVE EFFECT ON TOMATO MOSAIC DISEASE

ZHENG Gui-Bin

1. Xi'an Institute of Agriculture

Abstract: For the purpose of obtaining attenuated strain of TMV, pure virus isolated from tomato plants with mild symptom were treated with nitrous acid for 30, 40, 50, 60 minutes, as a result, 30 symptomless mutants had been obtained. In cross protection tests on tomato, the attenuated virus DN60-3 interfered more strongly with the severe mosaic strain, the streak strain and the necrosis strain occurred in Jinan (Shandong), whereas interfered weakly with the yellow mosaic strain and the yellow ringspot strain, both ...