THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY

YU Shan-Qian; ZHANG Ruo-Ping

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August 1987

YU Shan-Qian and ZHANG Ruo-Ping. THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY[J]. Virologica Sinica, 1987, 2(4).

Citation: YU Shan-Qian, ZHANG Ruo-Ping. THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY .VIROLOGICA SINICA, 1987, 2(4) : 69.

荧光底物在酶联免疫吸附试验中的应用

于善谦 ,
张若平

1.
复旦大学病毒研究室、上海

摘要

碱性磷酸酶底物,对硝基酚磷酸二钠(PNPP),在酶联免疫吸附试验(ELISA)中已普遍使用。本实验用一种荧光底物,4-甲基聚缴花酮磷酸酯做间接ELISA反应(FS-ELISA),同前者进行了比较。当用0.1ml的黄瓜花叶病毒(CMV),浓度为0.1ng/ml包被时,在紫外光下仍可看到反应所产生的荧光。FS-ELISA对CMV测定的敏感度比用PNPP底物时高100倍。最后对该方法用于定性和半定量检测抗原的问题进行了讨论。

THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY

1.
Virology Laboratory

Abstract

The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

THE APPLICATION OF FLUORESCENT SUBSTRATE IN ENZYME LINKED IMMUNOSORBENT ASSAY

1. Virology Laboratory

Abstract: The substrate, p-nitrophenolphosphate disodium (PNPP), of alkaline phosphatase has been commonly used in enzyme linked immunosorbent assay (ELISA).The fluorescent substrate, 4-methylumbelliferal phosphate, usedin the indirect ELISA procedure(FS-ELISA)was compared with the substrate PNPP used in same indirect ELISA procedure. The fluorescent reaction could be observed under the UV-light at 253.7nm emission wavelength when coating was done with 0.1ml suspension of cucumber mosaic virus at the minimum concentr...