BOVINE ROTAVIRUS ISOLATED FROM AN OUTBREAK OF ACUTE DIARRHEA IN NEWBORN CALVES

SU Cheng-Qin; TUN Yi-Lun; CHEN Yun-Hua; HE Li-Na; DONG Guang-Feng

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August 1987

SU Cheng-Qin, TUN Yi-Lun, CHEN Yun-Hua, HE Li-Na and DONG Guang-Feng. BOVINE ROTAVIRUS ISOLATED FROM AN OUTBREAK OF ACUTE DIARRHEA IN NEWBORN CALVES[J]. Virologica Sinica, 1987, 2(4).

Citation: SU Cheng-Qin, TUN Yi-Lun, CHEN Yun-Hua, HE Li-Na, DONG Guang-Feng. BOVINE ROTAVIRUS ISOLATED FROM AN OUTBREAK OF ACUTE DIARRHEA IN NEWBORN CALVES .VIROLOGICA SINICA, 1987, 2(4) : 79.

从一次新生小牛腹泻流行中分离到牛轮状病毒

1.
安徽省医学科学研究所
2.
安徽医科大学电镜室合肥

摘要

1985年3月下旬,合肥市某牛奶场发生了一次新生牛非细菌性腹泻流行。用MA104细胞从牛_1粪便标本中分离到一株牛轮状病毒。电镜检查为典型轮状病声形态。在MA104细胞上第三代开始呈现胞浆内颗粒状荧光并产生细胞病变(CPE)。PAGE核酸电泳图型与牛轮状病毒NCDV株完全一致。经ELISA鉴定属A群轮状病毒。

BOVINE ROTAVIRUS ISOLATED FROM AN OUTBREAK OF ACUTE DIARRHEA IN NEWBORN CALVES

1.
Anhui Province Institute of Medical sciences gefeiAnhui Medical University
2.
Hefei

Abstract

An outbreak of acute nonbacterial diarrhea ocurred among the newborn calves of a dairy in Hefei. A strain of bovine rotavirus was isolated using MA104 cells. The isolated virus appeared as typical rotavirus by electronmicroscopy, From the third passage it showed granulated fluorescence in the cytoplasm of MA104 cells and produced cytopathogenic effects(CPE).The RNA PAGE patterns of new isolated virus were indistinguishable from that of reference NCDV strain of bovine rotavirus. The new virus was identified ...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

BOVINE ROTAVIRUS ISOLATED FROM AN OUTBREAK OF ACUTE DIARRHEA IN NEWBORN CALVES

1. Anhui Province Institute of Medical sciences gefeiAnhui Medical University
2. Hefei

Abstract: An outbreak of acute nonbacterial diarrhea ocurred among the newborn calves of a dairy in Hefei. A strain of bovine rotavirus was isolated using MA104 cells. The isolated virus appeared as typical rotavirus by electronmicroscopy, From the third passage it showed granulated fluorescence in the cytoplasm of MA104 cells and produced cytopathogenic effects(CPE).The RNA PAGE patterns of new isolated virus were indistinguishable from that of reference NCDV strain of bovine rotavirus. The new virus was identified ...