Proliferation of Cells and Virus with Microcarrier Suspension Culture System

XIAO Cheng-Zu; ZHANG Ling-An; KONG Wei-Wei; WANG Hong-Xia

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April 1988

XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei and WANG Hong-Xia. Proliferation of Cells and Virus with Microcarrier Suspension Culture System[J]. Virologica Sinica, 1988, 3(2).

Citation: XIAO Cheng-Zu, ZHANG Ling-An, KONG Wei-Wei, WANG Hong-Xia. Proliferation of Cells and Virus with Microcarrier Suspension Culture System .VIROLOGICA SINICA, 1988, 3(2) : 160.

用微载体悬浮培养系统增殖细胞和病毒

1.
军事医学科学院微生物流行病研究所
2.
军事医学科学院微生物流行病研究所北京

摘要

我们用我院制备的MC-1型微载体成功地培养了BHK-13,BHK-21,和CHO-KI三株细胞。当微载体浓度为5mg/ml,细胞接种量为30×10~4细胞/ml左右时,一般在三天都可达到1×10~6细胞/ml。此时接种10~(-1)或10~(-2)的VSV,在37℃培养20-24小时,病毒产量可达到6LogTCTD_(50)/ml以上,其中以CHO-KI细胞的产量最高,它们与静止培养的细胞产量基本一致,也不亚于鸡胚细胞增殖的产量。目前用该系统增殖的VSV已用于IFN的研究工作中。实验的结果也表明,用该系统增殖其它病毒和制备疫苗,以及大量培养已引入重组IFN基因的CHO工程细胞也将是可行的。

Proliferation of Cells and Virus with Microcarrier Suspension Culture System

1.
Institute of Microbiology and Epidemiology
2.
Academy of Military Medical Sciences

Abstract

We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Proliferation of Cells and Virus with Microcarrier Suspension Culture System

1. Institute of Microbiology and Epidemiology
2. Academy of Military Medical Sciences

Abstract: We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...