MAN Yan-Gao. Inhibition of HSV-2 Replication in Vero Cells by Spleen and Lymphode Cells of Mice Infected with HSV-2 and Treated with Interfrons[J]. Virologica Sinica, 1988, 3(2).
Citation:
MAN Yan-Gao.
Inhibition of HSV-2 Replication in Vero Cells by Spleen and Lymphode Cells of Mice Infected with HSV-2 and Treated with Interfrons .VIROLOGICA SINICA, 1988, 3(2)
: 177.
脾及淋巴结细胞对单纯疱疹病毒Ⅱ型(HSV—2)复制的抑制作用
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摘要
为寻找激活细胞免疫的制剂及定量分析免疫细胞对HSV-2复制的影响,作者先以不同干扰素处理及HSV-2感染C_(57)BL/6J小鼠,而后取其脾及淋巴结制成细胞悬液,加至HSV-2感染的Vero单层细胞中,37℃混合培养48小时后,大多数Vero细胞无异常形态改变,但在此单层中,可见一些大小不等,形态不一的无细胞区。在此区边缘,可见脾及淋巴结细胞包绕有病理形态改变的Vero细胞;来加脾及淋巴结细胞的感染性单层则显示细胞变大、变园、融合等病理性形态学变化,但仍维持完整的单层状态。将以上培养物冻溶后测病毒效价发现,不同来源、不同数量的脾及淋巴结细胞对HSV-2的复制均有一定抑制作用,但以经HSV-2感染及γ干扰素处理的小鼠之脾细胞作用最强。在此细胞与靶细胞比例为100:1时,病毒复制受抑制的Log_(10)数可达4以上。该抑制作用与非特异性可溶性细胞毒性物质、营养代谢,pH、干扰素等因素无关。以上结果提示:采取适当方法激活并吸引免疫细胞抵达病毒感染部位,溶解感染细胞或破坏其与正常细胞的联系,可能会更有效地对抗通过细胞途径扩散的病毒性感染。
Inhibition of HSV-2 Replication in Vero Cells by Spleen and Lymphode Cells of Mice Infected with HSV-2 and Treated with Interfrons
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Abstract
In order to determine whether IFN treatment could make the cellular immune system more effective to against HSV-2 infection and develop a simple techniqe to quantitate the spread of HSV-2 infection in vitro, C57BL/6J mice were infected intravaginally with HSV-2 and treated witha recombinant mouse α/β or r interferon. 3 and 7 days after virus infection, the spleen and lymphnode of mice were aseptically removed. A single cell suspension was prepared and added onto the HSV-2 infected vero monolayers at a vario...
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