Studies of Electron Microscopy on the Virus Particles and the Inclusion-bodies by Ultrathin Sections for the Detection of Turnip Mosaic Virus

WEI Shi-Quan; GUO Ji-Xiang; ZHANG Ha-Yu

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April 1988

WEI Shi-Quan, GUO Ji-Xiang and ZHANG Ha-Yu. Studies of Electron Microscopy on the Virus Particles and the Inclusion-bodies by Ultrathin Sections for the Detection of Turnip Mosaic Virus[J]. Virologica Sinica, 1988, 3(2).

Citation: WEI Shi-Quan, GUO Ji-Xiang, ZHANG Ha-Yu. Studies of Electron Microscopy on the Virus Particles and the Inclusion-bodies by Ultrathin Sections for the Detection of Turnip Mosaic Virus .VIROLOGICA SINICA, 1988, 3(2) : 191.

芜菁花叶病毒粒体及寄主细胞内内含体的超薄切片电镜观察

1.
沈阳农业大学植保系
2.
中国科学院林业土壤研究所
3.
辽宁省理化测试中心沈阳

摘要

从沈阳地区分离到侵染大白菜(Brassicapekinensis)及萝卜(Raphanussativus)的芜菁花叶病毒(TurnipMosaicVirus,TuMV)纯化繁殖于大白菜(小白口)及萝卜(长白品种)上。经约25天采收纯化繁殖的毒原材料,进行病毒粗提纯,并做病毒粒子的电镜观察。看到病毒粒体较为疏散清晰,丝状,长度多分布在720nm之间。另将接种TuMV的感病寄主有关部位,切成小块经固定、系列脱水、环氮树脂包埋后,制作超薄切片并用电镜观察,看到病叶、病茎和病根韧皮部细胞内,均有清晰的风轮状、环状及带状的内含体,这是马铃薯Y病毒组(Potyvirus)成员特征之一。

Studies of Electron Microscopy on the Virus Particles and the Inclusion-bodies by Ultrathin Sections for the Detection of Turnip Mosaic Virus

1.
Shenyang Agri. Univ. ShenyangInstitute of Forestry and Soil Sciences
2.
China Academy of Scie. ces. Shenyang.Center Exp
3.
Station of Physics and Chemistry in Liaoning Province

Abstract

We have collected an isolate of turnip mosaic virus which infected Chincse cabbage(Brassica pekinensis) and radish(Raphanus sativus), and the isolate was identified by the plant virus researching group in Shenyang Agri. Univ. The pure isolate was propagated in netted green house in the University. The experimental host was Chinese cabbage(Xiao pei kuo) and radish(Longshaped white radish), and was inoculated by hand with the isolate and was used for specimens about three weeks later. For the electron microsc...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies of Electron Microscopy on the Virus Particles and the Inclusion-bodies by Ultrathin Sections for the Detection of Turnip Mosaic Virus

1. Shenyang Agri. Univ. ShenyangInstitute of Forestry and Soil Sciences
2. China Academy of Scie. ces. Shenyang.Center Exp
3. Station of Physics and Chemistry in Liaoning Province

Abstract: We have collected an isolate of turnip mosaic virus which infected Chincse cabbage(Brassica pekinensis) and radish(Raphanus sativus), and the isolate was identified by the plant virus researching group in Shenyang Agri. Univ. The pure isolate was propagated in netted green house in the University. The experimental host was Chinese cabbage(Xiao pei kuo) and radish(Longshaped white radish), and was inoculated by hand with the isolate and was used for specimens about three weeks later. For the electron microsc...