GUO Pei-Xuan. Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro[J]. Virologica Sinica, 1988, 3(2).
Citation:
GUO Pei-Xuan.
Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro .VIROLOGICA SINICA, 1988, 3(2)
: 198.
Novobiocin、Coumermycin A、Oxolinic acid、Nalidixic acid、溴化乙锭和ATP类似物对细菌病毒φ29细胞外DNA包装的抑制作用
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1.
华南农业大学 牧医系
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2.
微生物学教研室
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3.
广州美国纽约州卫生部研究中心病毒遗传室研究员
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摘要
四种抗菌素Novobiocin、CoumermycinA、Oxolinicacid和Nalidixicacid以及化合物溴化乙锭(Ethidiumbromide)和ATP类似物γ—S—ATP能抑制噬菌体φ29DNA的细胞外包装。达到50%抑制作用的药物浓度(不包括ATP类似物)分别为每毫升5、90、167、380和1.3微克。当ATP类似物加入完全纯化的φ29胞外DNA包装系统时,DNA包装的中间产物积蓄,DNA包装流产,表明DNA装壳的整个过程均需ATP的参与。这一发现为抗病毒药物的研究提供另一条线索。本文并对φ29DNA包装的可能机制进行讨论。
Inhibition of Novobiocin, Coumermycin A, Oxolinic Acid, Nalidixic Acid, Ethidium Bromide and ATP Analogue on the Packaging of Φ29 DNA in Vitro
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Abstract
Novobiocin, Coumermycin A, Oxolinic acid, Nalidixic acid, ethidium bromide and ATP analogue γ-S-ATP inhibited the DNA packaging of bacteriophage Φ29 in vitro. Besides ATP analogue, the concentration of the chemicals for 50% inhibition were 5, 90, 167, 380 and 1.3μg per ml, respectively. The intermediates of DNA packaging accumulated when the ATP analogue was added to the defined in vitro DNA packaging system, indicating that ATP was required for the entire process of DNA translocation. This finding provides...
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References
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Proportional views
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