CAO Zong-Li, CHOU Su-Ying, DONG Jie-De, CHEN Chen-Hua, ZENG Li, YU Xiu-Beng and SONG Yuan-Di. Transformation of Human Embryonic Lung Cells by Ultraviolet Irradiation Inactivated Herpes Simplex Virus Type 2[J]. Virologica Sinica, 1988, 3(3).
Citation: CAO Zong-Li, CHOU Su-Ying, DONG Jie-De, CHEN Chen-Hua, ZENG Li, YU Xiu-Beng, SONG Yuan-Di. Transformation of Human Embryonic Lung Cells by Ultraviolet Irradiation Inactivated Herpes Simplex Virus Type 2 .VIROLOGICA SINICA, 1988, 3(3) : 265.

单纯疱疹病毒Ⅱ型使人胚肺细胞转化的实验研究

  • 用紫外线灭活的单纯疱疹病毒Ⅱ型使人胚肺细胞发生转化,获得三系转化细胞。转化细胞呈多角形,似上皮细胞。细胞形态符合瘤细胞特征。细胞染色体数目明显增多,呈超二倍体型。体外培养时,细胞长满单层后极易堆集重叠生长成锥体细胞群,提示细胞的生长接触抑制消失。用间接免疫酶试验证实转化细胞内存在HSV特异抗原。HSV抗原在第51代转化细胞内仍持续存在。

Transformation of Human Embryonic Lung Cells by Ultraviolet Irradiation Inactivated Herpes Simplex Virus Type 2

  • Three transformed human cell lines were obtained after infection of human embryo lung cells with ultraviolet irradiation inactivated HSV-2. The transformed cells appear polygonal resembling epithelial cells. They are morphologically compatible with neoplastic cells, Chromosome examination showed hyperdiploid karyotype. In vitro, afftcr eonfluency of monolayers they continue to grow into pyramidal masses indicating loss of contact inhibition. Indirect immunoenzymatic test revealed the presence of HSV antigen...

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    Transformation of Human Embryonic Lung Cells by Ultraviolet Irradiation Inactivated Herpes Simplex Virus Type 2

    • 1. Department of Microbiology
    • 2. Shandong Medical University

    Abstract: Three transformed human cell lines were obtained after infection of human embryo lung cells with ultraviolet irradiation inactivated HSV-2. The transformed cells appear polygonal resembling epithelial cells. They are morphologically compatible with neoplastic cells, Chromosome examination showed hyperdiploid karyotype. In vitro, afftcr eonfluency of monolayers they continue to grow into pyramidal masses indicating loss of contact inhibition. Indirect immunoenzymatic test revealed the presence of HSV antigen...

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