Studies on Monoclonal Antibodies to Hepatitis A Virus

LI Cheng-Meng; YAN Da-Chang; WANG Hong-Xi; LIU Li-Hua; CHEN Dan-Lin; ZENG Xi-Hua; BI Xiao; CHEN Li-Li

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June 1988

LI Cheng-Meng, YAN Da-Chang, WANG Hong-Xi, LIU Li-Hua, CHEN Dan-Lin, ZENG Xi-Hua, BI Xiao and CHEN Li-Li. Studies on Monoclonal Antibodies to Hepatitis A Virus[J]. Virologica Sinica, 1988, 3(3).

Citation: LI Cheng-Meng, YAN Da-Chang, WANG Hong-Xi, LIU Li-Hua, CHEN Dan-Lin, ZENG Xi-Hua, BI Xiao, CHEN Li-Li. Studies on Monoclonal Antibodies to Hepatitis A Virus .VIROLOGICA SINICA, 1988, 3(3) : 272.

抗甲型肝炎病毒单克隆抗体(抗-HAV McAb)的研究

1.
四川省卫生防疫站病毒性疾病控制科
2.
四川省卫生防疫站病毒性疾病控制科成都

摘要

应用细胞融合技术成功地建立了3株持续高效分泌抗-HAV McAb的杂交瘤细胞株(AG_3,AD_2和AE_8株),所得腹水中的抗-HAV滴度达1:128000～1:1024000。这3株McAb都能与粪便提取HAV和细胞培养HAV反应,且这种反应均能被甲肝病人恢复期血清阻断。这些McAb作用于HAV的不同抗原位点,其中AE_8 McAb具有中和HAV感染性的能性。这些McAb作包被抗体检测HAV时,所测的HAV滴度较人抗体作包被时测得的滴度高2～8倍。将AE_8 McAb标记酶后,用以检测Abbott药盒标化的50份甲肝IgM血清,49份结果一致,符合率98％。

Studies on Monoclonal Antibodies to Hepatitis A Virus

1.
Departmynt of Viral Disease Control
2.
Health and Antiepidemic Centre of Sichuan Province

Abstract

Three strain hybridomas (AG3, AD2, AE8) were developed by cell fusion technique. They secreted stably MeAbs against HAV with the titer of 1: 128000—1: 1024000 in ascites fluid by ELISA. All of these McAbs are of positive reaction on HAV derived from patient s stools and cell culture, and this reaction can be inhibited by human anti HAV positive sera. The results of cell neutralization test and cross-blocking ELISA show that AE8 McAb is able to neutralize HAV infectivity, and that these McAbs recognize diffe...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Studies on Monoclonal Antibodies to Hepatitis A Virus

1. Departmynt of Viral Disease Control
2. Health and Antiepidemic Centre of Sichuan Province

Abstract: Three strain hybridomas (AG3, AD2, AE8) were developed by cell fusion technique. They secreted stably MeAbs against HAV with the titer of 1: 128000—1: 1024000 in ascites fluid by ELISA. All of these McAbs are of positive reaction on HAV derived from patient s stools and cell culture, and this reaction can be inhibited by human anti HAV positive sera. The results of cell neutralization test and cross-blocking ELISA show that AE8 McAb is able to neutralize HAV infectivity, and that these McAbs recognize diffe...