Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses

WEN Ping; FANG Yi-Lan

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June 1988

WEN Ping and FANG Yi-Lan. Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses[J]. Virologica Sinica, 1988, 3(3).

Citation: WEN Ping, FANG Yi-Lan. Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses .VIROLOGICA SINICA, 1988, 3(3) : 278.

DNA斑点杂交法在单纯疱疹病毒检测及分型中的应用

温平 ,
房益兰

1.
西安医科大学微生物学教研室
2.
西安医科大学微生物学教研室西安

摘要

本文从HSV-2(333)DNA BgLⅡH_2片段中筛选出HSV型共同性(7.8kb)及HSV-2型特异性(3.0kb)DNA片段,经~(32)P标记后作为探针用斑点杂交法对4株HSV标准株及24株疑为HSV分离株进行了检测和分型。结果能准确地将2株HSV-1及2株HSV-2标准株区分开,并确定24株疑HSV分离株中21株为HSV。这些结果与血清学鉴定结果一致,且HSV-2型特异性DNA探针的HSV-2检出率高于McAb。

Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses

WEN Ping ,
FANG Yi-Lan

1.
Department of Microbiology
2.
Xian Medical Univcrsity

Abstract

HSV type-common(7.8Kb)and HSV-2type-specific(3.0kb)DNA fragments were selected out from cloned HSV-2 (333) DNA Bgl Ⅱ H_2 fragment, and labeled with ~(32)P as probes to detect and type 4 HSV standard strains and 24 cervical viral isolates by dot-blot hybrization. In the results, 2HSV-1 and 2 HSV-2 were unequivocally differentiated, and 21 out of 24 isolates were identified as HSV, in which 3 were typed as HSV-1 and 18 as HSV-2. These results were consistent with those of ELISA, morover, the identification ra...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Application of Dot-blot DNA Hybridization in Detection and Typing of Herpes Simplex Viruses

WEN Ping ,
FANG Yi-Lan

1. Department of Microbiology
2. Xian Medical Univcrsity

Abstract: HSV type-common(7.8Kb)and HSV-2type-specific(3.0kb)DNA fragments were selected out from cloned HSV-2 (333) DNA Bgl Ⅱ H_2 fragment, and labeled with ~(32)P as probes to detect and type 4 HSV standard strains and 24 cervical viral isolates by dot-blot hybrization. In the results, 2HSV-1 and 2 HSV-2 were unequivocally differentiated, and 21 out of 24 isolates were identified as HSV, in which 3 were typed as HSV-1 and 18 as HSV-2. These results were consistent with those of ELISA, morover, the identification ra...