Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A

ZHENG Ji-Shan; LIN Jian-Wen; LI Fa-Qing; ZHENG Qi-Ge; LE Mei-Zhao

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June 1988

ZHENG Ji-Shan, LIN Jian-Wen, LI Fa-Qing, ZHENG Qi-Ge and LE Mei-Zhao. Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A[J]. Virologica Sinica, 1988, 3(3).

Citation: ZHENG Ji-Shan, LIN Jian-Wen, LI Fa-Qing, ZHENG Qi-Ge, LE Mei-Zhao. Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A .VIROLOGICA SINICA, 1988, 3(3) : 285.

从人外周血单个核白细胞分离甲型肝炎病毒

1.
南京军区军事医学研究所
2.
南京市传染病医院
3.
南京军区第八十一医院南京

摘要

选择HAV-Ag免疫荧光阳性的人血单个核白细胞标本,冻融后接种PLC/PRF/5细胞,分离到两株HAV(NJ-3和H-1株),并经血清学和形态学等鉴定证实。本实验提示:HAV不仅游离存在于血浆内,而且可与血白细胞相伴随。采用免疫荧光法筛选白细胞标本作接种物,方法简单,可作为分离HAV的新材料的来源。

Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A

1.
Institute of Military Medicine
2.
Nanjing Command
3.
PLA

Abstract

HAAg were detected from the samples of peripheral blood mononuelear leukocytesof patients with hepatitis A using IF technique. The positive samples weer frozen and thawed 3 times and inoculated into PLC/PRF/5 cell cultures. Two strains of HAV were isolated, The isolates proved to be HAV based on specific activity in serologic tests and morphology. The results of our studies suggested that HAV be present not only in the serum freely but also associated with leukocytes. It is a simple method to screen the sam...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Isolation of Hepatitis A Virus in Vitro Directly from Peripheral Blood Mononuclear Cell of Patients with Hepatitis A

1. Institute of Military Medicine
2. Nanjing Command
3. PLA

Abstract: HAAg were detected from the samples of peripheral blood mononuelear leukocytesof patients with hepatitis A using IF technique. The positive samples weer frozen and thawed 3 times and inoculated into PLC/PRF/5 cell cultures. Two strains of HAV were isolated, The isolates proved to be HAV based on specific activity in serologic tests and morphology. The results of our studies suggested that HAV be present not only in the serum freely but also associated with leukocytes. It is a simple method to screen the sam...