Research on a Strain of Carnation Mottle Virus Occurring in China Ⅰ. Host Range, Purification and Serology

YU Jia-Lin; LIU Yi

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June 1988

YU Jia-Lin and LIU Yi. Research on a Strain of Carnation Mottle Virus Occurring in China Ⅰ. Host Range, Purification and Serology[J]. Virologica Sinica, 1988, 3(3).

Citation: YU Jia-Lin, LIU Yi. Research on a Strain of Carnation Mottle Virus Occurring in China Ⅰ. Host Range, Purification and Serology .VIROLOGICA SINICA, 1988, 3(3) : 300.

我国发生的一种香石竹斑驳病毒株系研究Ⅰ型寄主范围、提纯及血清学特性

于嘉林 ,
刘仪

1.
北京农业大学
2.
北京农业大学植保系北京
3.
植保系北京

摘要

北京、上海栽培香石竹上发生的一种香石竹斑驳病毒BS株系(Carnation Mottle Virus-BS Strain),人工接种可局部侵染苋色藜,墙生藜,千日红及番杏,有时可系统侵染昆诺阿藜,分别造成坏死斑或褪绿斑,不侵染所试其它2科5种植物。单斑分离接种昆诺阿黎做为提纯材料,经PEG(分子量6,000)沉淀,差速离心及蔗糖密度梯度离心,病毒提纯量约140毫克/公斤鲜组织。病毒颗粒呈等轴多面体,直径为33毫微米。提纯物紫外扫描呈典型病毒核蛋白吸收曲线,A260/A280值为1.54,核酸含量约20％。提纯病毒免疫家兔所得抗血清试管沉淀效价为1:4096,琼脂糖双扩散效价可达1:1024。此株系血清学反应与英国香石竹斑驳病毒B-362株系呈部分同源性。

Research on a Strain of Carnation Mottle Virus Occurring in China Ⅰ. Host Range, Purification and Serology

YU Jia-Lin ,
LIU Yi

1.
Plant Protection Dcpartmcnt of Beijing Agricultural University
2.
Beijing

Abstract

Carnation mottle virus BS strain, which was isolated from carnation plants grown in gardens in Beijing and Shanghai of China,infected Chenopodium murale, C. amarantieolor, Gomphrena globosa and Tetragonia expansa locally, C.quinoa systemically sometimes and induced necrotic spots or chlorotic lesions on these hosts respectively. But other five species plants of two families tested were not infected. After two cycles single lesion isolation, the virus yield purified from C. quinoa by PEG(MW 6,000) precipitat...

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Research on a Strain of Carnation Mottle Virus Occurring in China Ⅰ. Host Range, Purification and Serology

YU Jia-Lin ,
LIU Yi

1. Plant Protection Dcpartmcnt of Beijing Agricultural University
2. Beijing

Abstract: Carnation mottle virus BS strain, which was isolated from carnation plants grown in gardens in Beijing and Shanghai of China,infected Chenopodium murale, C. amarantieolor, Gomphrena globosa and Tetragonia expansa locally, C.quinoa systemically sometimes and induced necrotic spots or chlorotic lesions on these hosts respectively. But other five species plants of two families tested were not infected. After two cycles single lesion isolation, the virus yield purified from C. quinoa by PEG(MW 6,000) precipitat...