Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection

DING Qing-Quan; CHEN Shao-Dun; KE Li-Hua

PDF
Cite
Share

facebook

twitter

google

linkedin

February 1989

DING Qing-Quan, CHEN Shao-Dun and KE Li-Hua. Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection[J]. Virologica Sinica, 1989, 4(1).

Citation: DING Qing-Quan, CHEN Shao-Dun, KE Li-Hua. Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection .VIROLOGICA SINICA, 1989, 4(1) : 16.

α—(32)P HBV DNA探针的制备及其在血清检测中的应用

1.
中国科学院武汉病毒研究所
2.
中国科学院武汉病毒研究所武汉

摘要

采用缺口转移(Nick-translation)方法标记克隆化的乙型肝炎病毒(HBV)DNA制备探针,能特异地与HBV DNA杂交,在本实验条件下,其最低检测量为0.2pg,同位素参入率为23.5—67.0％,使用100μCi ~(32)P dCTP,比放射活性为10~8cpm//μg DNA左右。临床血清检测统计分析表明:HBV DNA与HBsAg、HBeAg、HBcAg、DNAP的阳性符合率分别为53.3％、69.7％、78.6％、81.7％,其灵敏性和特异性均比免疫学检测法高。
- 乙型肝炎病毒
- , 探针
- , 缺口转移
- , 血清检测

Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection

1.
Wuhan Institute of Virology
2.
Academia Sinica

Abstract

Hepatitis B virus(HBV) DNA purified from the cloned plasmid pHBV_1 and labelled by nick-translation with α-~(32)p dCTP was as probe, which can be hybridized specifically with HBV DNA. The minimum amount about 0.2pg of DNA has been detected by high specific activity probes. The range of the isotope incorporation rates were 23.5-67.0 per cent. The probes s specific activities were 1-1.50×10~8cpm/μg DNA. According to clinical statistics the positive proportions of HBV DNA Correspond to 53.3% 69.7% 78.6% and 81...
- Hepatitis B virus
- , Probe
- , Nick translation
- , Serum detection

References
Proportional views

PDF

Article Metrics

Article views(3536) PDF downloads(791) Cited by(0)

Proportional views

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Preparation of α-~(32)P HBV DNA Probe and its Application in the Serum Detection

1. Wuhan Institute of Virology
2. Academia Sinica

Abstract: Hepatitis B virus(HBV) DNA purified from the cloned plasmid pHBV_1 and labelled by nick-translation with α-~(32)p dCTP was as probe, which can be hybridized specifically with HBV DNA. The minimum amount about 0.2pg of DNA has been detected by high specific activity probes. The range of the isotope incorporation rates were 23.5-67.0 per cent. The probes s specific activities were 1-1.50×10~8cpm/μg DNA. According to clinical statistics the positive proportions of HBV DNA Correspond to 53.3% 69.7% 78.6% and 81...